9ZEB

Cryo-EM structure of the TREX-2.1 complex (Thp3/Csn12/Sem1) bound to the DEAD-box ATPase Sub2

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein THP3	polymer	336	39251.9	1	UniProt (Q12049)	Saccharomyces cerevisiae (brewer's yeast)	THO-related protein 3
2	B (B)	Cop9 signalosome complex subunit 12	polymer	429	50099.9	1	UniProt (P47130)	Saccharomyces cerevisiae (brewer's yeast)
3	C (C)	26S proteasome complex subunit SEM1	polymer	93	10827.6	1	UniProt (O94742)	Saccharomyces cerevisiae (brewer's yeast)
4	D (D)	ATP-dependent RNA helicase SUB2	polymer	451	50901.5	1	UniProt (Q07478)	Saccharomyces cerevisiae (brewer's yeast)	Suppressor of BRR1 protein 2
5	E (D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

A: 138 - 470 (UniProt: Q12049)

PDB	External Database	Details
Gly 135	-	expression tag
Ala 136	-	expression tag
Met 137	-	expression tag

B: 1 - 423 (UniProt: P47130)

PDB	External Database	Details
Gly -5	-	expression tag
Ala -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag

C: 1 - 89 (UniProt: O94742)

PDB	External Database	Details
Met -3	-	initiating methionine
Ala -2	-	expression tag
Asp -1	-	expression tag
Leu 0	-	expression tag

D: 1 - 446 (UniProt: Q07478)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
A1amm 125	Asp 125	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151081.0
Non-Polymers*	Number of molecules	1
	Total formula weight	427.2
All*	Total formula weight	151508.2

*Water molecules are not included.