9ZE3
Cryo-EM structure of the endogenous U2/branchpoint spliceosomal complex (Distal DHX15 state)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RNA-binding protein 5 | polymer | 824 | 93410.1 | 1 | UniProt (P52756) | Homo sapiens (human) | Protein G15,Putative tumor suppressor LUCA15,RNA-binding motif protein 5,Renal carcinoma antigen NY-REN-9 |
| 2 | B (B) | ATP-dependent RNA helicase DHX15 | polymer | 795 | 91065.8 | 1 | UniProt (O43143) | Homo sapiens (human) | ATP-dependent RNA helicase #46,DEAH box protein 15,Splicing factor Prp43,hPrp43 |
| 3 | C (C) | U2 snRNP-associated SURP motif-containing protein | polymer | 1029 | 118503.3 | 1 | UniProt (O15042) | Homo sapiens (human) | 140 kDa Ser/Arg-rich domain protein,U2-associated protein SR140 |
| 4 | D (2) | U2 snRNA | polymer | 37 | 11785.0 | 1 | Homo sapiens (human) | ||
| 5 | E (H) | PHD finger-like domain-containing protein 5A | polymer | 110 | 12427.5 | 1 | UniProt (Q7RTV0) | Homo sapiens (human) | PHD finger-like domain protein 5A,Splicing factor 3B-associated 14 kDa protein,SF3b14b |
| 6 | F (R) | Pre-mRNA | polymer | 44 | 13447.4 | 1 | Homo sapiens (human) | ||
| 7 | G (B1) | Splicing factor 3B subunit 1 | polymer | 1304 | 146024.9 | 1 | UniProt (O75533) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 155 kDa subunit,SF3b155,Spliceosome-associated protein 155,SAP 155 |
| 8 | H (B2) | Splicing factor 3B subunit 2 | polymer | 895 | 100377.8 | 1 | UniProt (Q13435) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 145 kDa subunit,SF3b145,Spliceosome-associated protein 145,SAP 145 |
| 9 | I (B3) | Splicing factor 3B subunit 3 | polymer | 1217 | 135718.8 | 1 | UniProt (Q15393) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130 |
| 10 | J (B4) | Splicing factor 3B subunit 4 | polymer | 424 | 44436.6 | 1 | UniProt (Q15427) | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 49 kDa subunit,Spliceosome-associated protein 49,SAP 49 |
| 11 | K (B5) | Splicing factor 3B subunit 5 | polymer | 86 | 10149.4 | 1 | UniProt (Q9BWJ5) | Homo sapiens (human) | SF3b5,Pre-mRNA-splicing factor SF3b 10 kDa subunit |
| 12 | L (B6) | Splicing factor 3B subunit 6 | polymer | 125 | 14606.9 | 1 | UniProt (Q9Y3B4) | Homo sapiens (human) | Pre-mRNA branch site protein p14,SF3b 14 kDa subunit,SF3B14a,Spliceosome-associated protein,14-kDa,Splicing factor 3b,subunit 6,14kDa |
| 13 | M (A1) | Splicing factor 3A subunit 1 | polymer | 793 | 88991.1 | 1 | UniProt (Q15459) | Homo sapiens (human) | SF3a120,Spliceosome-associated protein 114,SAP 114 |
| 14 | N (A2) | Splicing factor 3A subunit 2 | polymer | 464 | 49327.4 | 1 | UniProt (Q15428) | Homo sapiens (human) | SF3a66,Spliceosome-associated protein 62,SAP 62 |
| 15 | O (A3) | Splicing factor 3A subunit 3 | polymer | 501 | 58934.8 | 1 | UniProt (Q12874) | Homo sapiens (human) | SF3a60,Spliceosome-associated protein 61,SAP 61 |
| 16 | P, Q, R (H) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) PubChem (32051) |
Sequence modifications
A: 1 - 815 (UniProt: P52756)
| PDB | External Database | Details |
|---|---|---|
| Met 816 | - | expression tag |
| Asp 817 | - | expression tag |
| Tyr 818 | - | expression tag |
| Lys 819 | - | expression tag |
| Asp 820 | - | expression tag |
| Asp 821 | - | expression tag |
| Asp 822 | - | expression tag |
| Asp 823 | - | expression tag |
| Lys 824 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 989206.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 989403.1 |
