9ZCS

Crystal structure of HOPS subunits Vps33 and Vps16 in complex with the Nyv1 and Vam3 SNARE motifs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Small conjugating protein ligase-like protein	polymer	696	77119.6	1	UniProt (G0SCM5) Pfam (PF00995)	Thermochaetoides thermophila DSM 1495	SM protein Vps33
2	B (B)	Vacuolar protein sorting-associated protein 16	polymer	274	31252.7	1	UniProt (G0S6M7) Pfam (PF04840)	Thermochaetoides thermophila DSM 1495
3	C (C)	Nyv1 SNARE motif	polymer	67	7570.6	1	UniProt (G0S5G3) Pfam (PF00957)	Thermochaetoides thermophila DSM 1495
4	D (D)	SNAP receptor-like protein	polymer	51	5669.2	1	UniProt (G0S236)	Thermochaetoides thermophila DSM 1495	Vam3

Sequence modifications

A: 0 - 667 (UniProt: G0SCM5)

PDB	External Database	Details
Met -28	-	initiating methionine
Lys -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Gly -19	-	expression tag
Ala -18	-	expression tag
Ala -17	-	expression tag
Gly -16	-	expression tag
Thr -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Lys -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag

B: 521 - 791 (UniProt: G0S6M7)

PDB	External Database	Details
Met 518	-	initiating methionine
Gly 519	-	expression tag
Ser 520	-	expression tag
Arg 672	-	insertion
Met 673	-	insertion
Gln 674	-	insertion
Glu 675	-	insertion
Thr 676	-	insertion
Phe 677	-	insertion
Glu 678	-	insertion
Arg 679	-	insertion
Asp 680	-	insertion
Leu 681	-	insertion
Thr 682	-	insertion
Asp 683	-	insertion
Ser 684	-	insertion
Phe 685	-	insertion
Val 686	-	insertion
Gly 687	-	insertion
Ser 689	-	insertion
Val 690	-	insertion

C: 148 - 213 (UniProt: G0S5G3)

PDB	External Database	Details
Gly 146	-	expression tag
Ser 147	-	expression tag

D: 181 - 229 (UniProt: G0S236)

PDB	External Database	Details
Gly 179	-	expression tag
Ser 180	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	121612.1
All*	Total formula weight	121612.1

*Water molecules are not included.