9Z7Q
Cryo-EM Structure of the Type III-Bv CRISPR Complex from Dissulfurispira thermophila bound to a crRNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Type III-B CRISPR-associated protein Cas10/Cmr2 | polymer | 612 | 70288.9 | 1 | UniProt (A0A7G1H3Q2) | Dissulfurispira thermophila | |
| 2 | B (B) | Type III-B CRISPR module-associated protein Cmr3 | polymer | 360 | 40201.4 | 1 | UniProt (A0A7G1H1A4) | Dissulfurispira thermophila | |
| 3 | C, D, E (C, D, E) | Type III-B CRISPR module RAMP protein Cmr4 | polymer | 315 | 34966.7 | 3 | UniProt (A0A7G1H376) | Dissulfurispira thermophila | |
| 4 | F, G (F, G) | CRISPR type III-B/RAMP module-associated protein Cmr5 | polymer | 140 | 15953.4 | 2 | UniProt (A0A7G1H353) | Dissulfurispira thermophila | |
| 5 | H (H) | CSD domain-containing protein Cmr6 | polymer | 376 | 42396.6 | 1 | UniProt (A0A7G1H339) | Dissulfurispira thermophila | |
| 6 | I (N) | RNA (38-MER) | polymer | 38 | 12248.4 | 1 | Escherichia phage MS2 | ||
| 7 | J (I) | Cmr1-MntA | polymer | 438 | 49646.6 | 1 | UniProt (A0A7G1H1S1) | Dissulfurispira thermophila | |
| 8 | K (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) PubChem (32051) |
Sequence modifications
A: 1 - 596 (UniProt: A0A7G1H3Q2)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Gly -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 351588.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 351653.9 |
