9Z4E

Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CATGAGTCAT and loaded with mutated Bzip region of GCN4 transcription factor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (D, F, E)	General control transcription factor GCN4	polymer	29	3422.1	3	UniProt (P03069) Pfam (PF07716)	Saccharomyces cerevisiae	Amino acid biosynthesis regulatory protein,General control protein GCN4
2	D (A)	DNA (31-MER)	polymer	31	9472.1	1		Escherichia coli
3	E (B)	DNA (31-MER)	polymer	31	9592.2	1		Escherichia coli
4	F (C)	Replication initiation protein	polymer	263	30749.1	1	UniProt (P03856)	Escherichia coli	Protein E,Protein rep,Protein F4, Replication Initiator protein RepE54
5	G, H, I (A, B, C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

D, F, E: 1 - 29 (UniProt: P03069)

PDB	External Database	Details
Lys 26	-	insertion
Cys 29	Leu 253	engineered mutation

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	60079.6
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	60152.5

*Water molecules are not included.