9Z3Q

Cryo-EM structure of KSHV glycoprotein gHgL in complex with MLKH3 and MLKH10 FABs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MLKH10 Light chain FV	polymer	112	12427.8	1		Mus musculus (mouse)
2	B (B)	MLKH10 Heavy chain FV	polymer	121	13144.4	1		Mus musculus (mouse)
3	C (C)	MLKH3 Light chain FV	polymer	107	11538.8	1		Mus musculus (mouse)
4	D (D)	MLKH3 Heavy chain FV	polymer	122	13088.5	1		Mus musculus (mouse)
5	E (E)	Envelope glycoprotein H	polymer	728	80917.5	1	UniProt (F5HAK9) Pfam (PF02489) Pfam (PF17488)	Human gammaherpesvirus 8	gH
6	F (F)	Envelope glycoprotein L	polymer	176	18815.8	1	UniProt (F5HDB7) Pfam (PF11108)	Human gammaherpesvirus 8	gL

Sequence modifications

E: 23 - 698 (UniProt: F5HAK9)

PDB	External Database	Details
Met -3	-	initiating methionine
Asp -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Lys 1	-	expression tag
Arg 2	-	expression tag
Gly 3	-	expression tag
Leu 4	-	expression tag
Cys 5	-	expression tag
Cys 6	-	expression tag
Val 7	-	expression tag
Leu 8	-	expression tag
Leu 9	-	expression tag
Leu 10	-	expression tag
Cys 11	-	expression tag
Gly 12	-	expression tag
Ala 13	-	expression tag
Val 14	-	expression tag
Phe 15	-	expression tag
Val 16	-	expression tag
Ser 17	-	expression tag
Pro 18	-	expression tag
Ser 19	-	expression tag
Ala 20	-	expression tag
Ser 21	-	expression tag
Ala 22	-	expression tag
Gly 699	-	expression tag
Ser 700	-	expression tag
Gly 701	-	expression tag
Ser 702	-	expression tag
Gly 703	-	expression tag
His 704	-	expression tag
His 705	-	expression tag
His 706	-	expression tag
His 707	-	expression tag
His 708	-	expression tag
His 709	-	expression tag
Gly 710	-	expression tag
Leu 711	-	expression tag
Asn 712	-	expression tag
Asp 713	-	expression tag
Ile 714	-	expression tag
Phe 715	-	expression tag
Glu 716	-	expression tag
Ala 717	-	expression tag
Gln 718	-	expression tag
Lys 719	-	expression tag
Ile 720	-	expression tag
Glu 721	-	expression tag
Trp 722	-	expression tag
His 723	-	expression tag
Glu 724	-	expression tag

F: 21 - 167 (UniProt: F5HDB7)

PDB	External Database	Details
Met -8	-	initiating methionine
Asp -7	-	expression tag
Ala -6	-	expression tag
Met -5	-	expression tag
Lys -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Leu -1	-	expression tag
Cys 0	-	expression tag
Cys 1	-	expression tag
Val 2	-	expression tag
Leu 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Cys 6	-	expression tag
Gly 7	-	expression tag
Ala 8	-	expression tag
Val 9	-	expression tag
Phe 10	-	expression tag
Val 11	-	expression tag
Ser 12	-	expression tag
Pro 13	-	expression tag
Ser 14	-	expression tag
Ala 15	-	expression tag
Ser 16	-	expression tag
Asp 17	-	expression tag
Gly 18	-	expression tag
Ile 19	-	expression tag
Gln 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	149932.7
All*	Total formula weight	149932.7

*Water molecules are not included.