9YZQ

Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TGATGAGCAG and loaded with Engrailed homeodomain enhanced Green fluorescent protein fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (31-MER)	polymer	31	9496.1	1		Escherichia coli
2	B (B)	DNA (31-MER)	polymer	31	9567.2	1		Escherichia coli
3	C (C)	Replication initiation protein	polymer	263	30749.1	1	UniProt (P03856)	Escherichia coli	Protein E,Protein rep,Protein F4, Replication Initiator protein RepE54
4	D (D)	Segmentation polarity homeobox protein engrailed,Green fluorescent protein	polymer	313	36124.8	1	UniProt (P02836) UniProt (P42212) Pfam (PF00046) Pfam (PF10525)	Drosophila melanogaster (fruit fly)
5	E, F (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	conflict

D: 2 - 60 (UniProt: P02836)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 61	-	linker
Ser 62	-	linker
Gln 63	-	linker
Phe 64	-	linker
Tyr 65	-	linker
Leu 66	-	linker
Asn 67	-	linker
Glu 68	-	linker

D: 69 - 307 (UniProt: P42212)

PDB	External Database	Details
Val 70	-	insertion
Leu 133	Phe 64	conflict
Thr 134	Ser 65	conflict
Leu 300	His 231	conflict
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	85937.1
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	85985.7

*Water molecules are not included.