9YZM

Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CTAATTAGGC and loaded with Even-skipped homeodomain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (31-MER)	polymer	31	9472.1	1		Escherichia coli
2	B (B)	DNA (31-MER)	polymer	31	9592.2	1		Escherichia coli
3	C (C)	Replication initiation protein	polymer	263	30749.1	1	UniProt (P03856)	Escherichia coli	Protein E,Protein rep,Protein F4, Replication Initiator protein RepE54
4	D, E (D, E)	Segmentation protein even-skipped	polymer	64	7853.0	2	UniProt (P06602) Pfam (PF00046)	Drosophila melanogaster (fruit fly)
5	F, G (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	conflict

D, E: 4 - 64 (UniProt: P06602)

PDB	External Database	Details
Gly 1	-	expression tag
Thr 2	-	expression tag
Gly 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	65519.3
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	65568.0

*Water molecules are not included.