9YZA

Isoreticular co-crystal 1 of protein variant Replication Initiator Protein REPE54 (L53G, Q54G, E55G) with symmetrical expanded duplex (31mer) containing insert sequence CCCGGCCGGA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (31-MER)	polymer	31	9459.1	1		Escherichia coli
2	B (B)	DNA (31-MER)	polymer	31	9610.2	1		Escherichia coli
3	C (C)	Replication initiation protein	polymer	263	30549.8	1	UniProt (P03856)	Escherichia coli	Protein E,Protein rep,Protein F4
4	D, E (B, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 53	Leu 53	engineered mutation
Gly 54	Gln 54	engineered mutation
Gly 55	Glu 55	engineered mutation
Pro 118	Arg 118	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	49619.0
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	49667.7

*Water molecules are not included.