9YY5

Crystal structure of P. furiosus SRP54 NG domain G225E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Signal recognition particle 54 kDa protein	polymer	316	34903.4	1	UniProt (Q8U070)	Pyrococcus furiosus	SRP54
2	B (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
4	D (A)	water	water		18.0	238	Chemie (HOH)

Sequence modifications

A: 1 - 295 (UniProt: Q8U070)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Glu 225	Gly 225	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34903.4
Non-Polymers*	Number of molecules	2
	Total formula weight	467.5
All*	Total formula weight	35371.0

*Water molecules are not included.