9YVP

Cryo-EM structure of the L900V;R993P;S1060R mutant mouse TRPM4 channel in an open state bound to NC1 and PI(4,5)P2.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (B, A, C, D)	Transient receptor potential cation channel subfamily M member 4	polymer	1229	137654.4	4	UniProt (Q7TN37)	Mus musculus (house mouse)	Calcium-activated non-selective cation channel 1,Long transient receptor potential channel 4,LTrpC-4,LTrpC4
2	E, H, K, N (B, A, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA) PubChem (271)
3	F, I, L, O (B, A, C, D)	[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate	non-polymer		1047.1	4	Chemie (PT5)
4	G, J, M, P (B, A, C, D)	Necrocide 1	non-polymer		365.5	4	Chemie (A1CY8)

Sequence modifications

B, A, C, D: 1 - 1213 (UniProt: Q7TN37)

PDB	External Database	Details
Val 900	Leu 900	engineered mutation
Pro 993	Arg 993	engineered mutation
Arg 1060	Ser 1060	engineered mutation
Leu 1214	-	expression tag
Val 1215	-	expression tag
Pro 1216	-	expression tag
Arg 1217	-	expression tag
Gly 1218	-	expression tag
Ser 1219	-	expression tag
Gly 1220	-	expression tag
Ala 1221	-	expression tag
Asp 1222	-	expression tag
Tyr 1223	-	expression tag
Lys 1224	-	expression tag
Asp 1225	-	expression tag
Asp 1226	-	expression tag
Asp 1227	-	expression tag
Asp 1228	-	expression tag
Lys 1229	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	550617.6
Non-Polymers*	Number of molecules	12
	Total formula weight	5810.5
All*	Total formula weight	556428.1

*Water molecules are not included.