9YRG
Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH undocked state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Myosin-7,General control transcription factor GCN4,Enhanced Green fluorescent protein | polymer | 1315 | 150656.5 | 2 | UniProt (P12883) UniProt (P03069) UniProt (P42212) | Homo sapiens (human) | Myosin heavy chain 7,Myosin heavy chain slow isoform,MyHC-slow,Myosin heavy chain,cardiac muscle beta isoform,MyHC-beta,Amino acid biosynthesis regulatory protein,General control protein GCN4 |
| 2 | C, D (C, D) | Myosin light chain 1/3, skeletal muscle isoform | polymer | 188 | 20620.5 | 2 | UniProt (P05977) | Mus musculus (house mouse) | MLC1/MLC3,MLC1F/MLC3F,Myosin light chain alkali 1/2,Myosin light chain A1/A2 |
| 3 | E, F (E, F) | Myosin regulatory light chain 11 | polymer | 169 | 18978.4 | 2 | UniProt (P97457) | Mus musculus (house mouse) | Fast skeletal myosin light chain 2,MLC2F,Myosin light chain 11,Myosin regulatory light chain 2,skeletal muscle isoform |
| 4 | G, I (A, B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 5 | H, J (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) PubChem (1061) |
Sequence modifications
A, B: 1 - 1016 (UniProt: P12883)
A, B: 1017 - 1048 (UniProt: P03069)
A, B: 1055 - 1292 (UniProt: P42212)
A, B: 1017 - 1048 (UniProt: P03069)
| PDB | External Database | Details |
|---|---|---|
| Gly 1049 | - | linker |
| Ser 1050 | - | linker |
| Gly 1051 | - | linker |
| Lys 1052 | - | linker |
| Leu 1053 | - | linker |
| Gly 1054 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Val 1055 | Met 1 | engineered mutation |
| Leu 1118 | Phe 64 | engineered mutation |
| Thr 1119 | Ser 65 | engineered mutation |
| Leu 1285 | His 231 | engineered mutation |
| Gly 1293 | - | expression tag |
| Leu 1294 | - | expression tag |
| Asn 1295 | - | expression tag |
| Asp 1296 | - | expression tag |
| Ile 1297 | - | expression tag |
| Phe 1298 | - | expression tag |
| Glu 1299 | - | expression tag |
| Ala 1300 | - | expression tag |
| Gln 1301 | - | expression tag |
| Lys 1302 | - | expression tag |
| Ile 1303 | - | expression tag |
| Glu 1304 | - | expression tag |
| Trp 1305 | - | expression tag |
| His 1306 | - | expression tag |
| Glu 1307 | - | expression tag |
| Asp 1308 | - | expression tag |
| Tyr 1309 | - | expression tag |
| Lys 1310 | - | expression tag |
| Asp 1311 | - | expression tag |
| Asp 1312 | - | expression tag |
| Asp 1313 | - | expression tag |
| Asp 1314 | - | expression tag |
| Lys 1315 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 380510.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1044.3 | |
| All* | Total formula weight | 381555.2 |
