9YNP

Cryo-EM structure of Escherichia coli transcription initiation complex with GpA and pseudouridimycin (PUM)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4)	Escherichia coli str. K-12 substr. MG1655	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2)	Escherichia coli str. K-12 substr. MG1655	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7)	Escherichia coli str. K-12 substr. MG1655	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800)	Escherichia coli str. K-12 substr. MG1655	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor RpoD	polymer	613	70352.2	1	UniProt (P00579)	Escherichia coli str. K-12 substr. MG1655	Sigma-70
6	G (G)	DNA (27-MER)	polymer	27	8421.4	1		Escherichia coli str. K-12 substr. MG1655
7	H (H)	DNA	polymer	21	6464.2	1		Escherichia coli str. K-12 substr. MG1655
8	I (I)	RNA (5'-R(*GP*A)-3')	polymer	2	629.5	1		Escherichia coli str. K-12 substr. MG1655
9	J, K (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	L (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
11	M (I)	(1S)-1,4-anhydro-5-[(N-carbamimidoylglycyl-N~2~-hydroxy-L-glutaminyl)amino]-5-deoxy-1-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-D-ribitol	non-polymer		486.4	1	Chemie (PUM)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	475421.9
Non-Polymers*	Number of molecules	4
	Total formula weight	641.6
All*	Total formula weight	476063.4

*Water molecules are not included.