9YMG

Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953)	Sus scrofa (pig)	Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain
2	B, E (B, E)	Tubulin beta chain	polymer	444	49793.7	2	UniProt (P02554) Pfam (PF00091) Pfam (PF03953)	Sus scrofa (pig)	Beta-tubulin
3	C, F (C, F)	Kinesin-like protein KIF18A, DARPin fusion protein	polymer	555	59769.4	2	UniProt (Q8NI77) Pfam (PF00225)	Homo sapiens (human)	Marrow stromal KIF18A,MS-KIF18A
4	G, N (A, D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
5	H, I, M, O, P... (A, C, D, F)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG) PubChem (888)
6	J (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	K, Q (B, E)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
8	L, R (C, F)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
9	T, U, V, W, X... (A, B, C, D, E...)	water	water		18.0	455	Chemie (HOH)

Sequence modifications

B, E: 1 - 444 (UniProt: P02554)

PDB	External Database	Details
Glu 433	Gln 433	conflict
Glu 434	Gly 434	conflict
Asp 435	Glu 435	conflict
Gly 437	Glu 437	conflict
Ala 440	Gly 440	conflict
Glu 443	Asp 443	conflict
Ala 444	Glu 444	conflict

C, F: 1 - 367 (UniProt: Q8NI77)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

C, F: 368 - 552 (PDB: 9YMG)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	319535.0
Non-Polymers*	Number of molecules	13
	Total formula weight	3153.1
All*	Total formula weight	322688.0

*Water molecules are not included.