9YLR

Nucleosome scFv complex with a nick at SHL +5.3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Histone H2A	polymer	124	13388.7	2	UniProt (P84051) Pfam (PF00125) Pfam (PF16211)	Drosophila melanogaster (fruit fly)
2	C, D (C, D)	Histone H2B	polymer	125	13897.3	2	UniProt (P02283) Pfam (PF00125)	Drosophila melanogaster (fruit fly)
3	E, F (E, F)	Histone H3	polymer	136	15405.0	2	UniProt (P02299) Pfam (PF00125)	Drosophila melanogaster (fruit fly)
4	G, H (G, H)	Histone H4	polymer	104	11521.6	2	UniProt (P84040) Pfam (PF15511)	Drosophila melanogaster (fruit fly)
5	I, J (I, J)	scFv20	polymer	274	29631.7	2		Mus musculus
6	K (K)	DNA (130-MER)	polymer	130	39859.4	1		synthetic construct
7	L (L)	DNA (23-MER)	polymer	23	7054.6	1		synthetic construct
8	M (M)	DNA (153-MER)	polymer	153	47497.3	1		synthetic construct

Sequence modifications

C, D: 3 - 124 (UniProt: P02283)

PDB	External Database	Details
Gly 0	-	expression tag
Met 1	-	expression tag
Ile 2	-	expression tag

E, F: 1 - 136 (UniProt: P02299)

PDB	External Database	Details
Ser 111	Cys 111	conflict

G, H: 3 - 104 (UniProt: P84040)

PDB	External Database	Details
Met 1	-	initiating methionine
Ile 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	262099.8
All*	Total formula weight	262099.8

*Water molecules are not included.