9YKW
Cryo-EM structure of double-loaded human UBA6-UBE2Z-Ub(t)/Ub(a) thioester mimetic complex.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ubiquitin-like modifier-activating enzyme 6 | polymer | 1016 | 114475.9 | 1 | UniProt (A0AVT1) | Homo sapiens (human) | Ubiquitin-activating enzyme 6,Monocyte protein 4,MOP-4,Ubiquitin-activating enzyme E1-like protein 2,E1-L2 |
| 2 | B, D (B, D) | Ubiquitin | polymer | 76 | 8576.8 | 2 | UniProt (P0CG48) | Homo sapiens (human) | |
| 3 | C (C) | Ubiquitin-conjugating enzyme E2 Z | polymer | 262 | 29909.0 | 1 | UniProt (Q9H832) | Homo sapiens (human) | E2 ubiquitin-conjugating enzyme Z,Uba6-specific E2 conjugating enzyme 1,Use1,Ubiquitin carrier protein Z,Ubiquitin-protein ligase Z |
| 4 | E (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) |
Sequence modifications
C: 93 - 354 (UniProt: Q9H832)
| PDB | External Database | Details |
|---|---|---|
| Ser 100 | Cys 100 | engineered mutation |
| Lys 182 | Tyr 182 | engineered mutation |
| Arg 186 | Lys 186 | engineered mutation |
| Ser 261 | Cys 261 | engineered mutation |
| Ser 263 | Cys 263 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 161538.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 660.0 | |
| All* | Total formula weight | 162198.5 |
