9YKV

Cryo-EM structure of double-loaded human UBA6-UBE2Z-FAT10(t)/FAT10(a) thioester mimetic complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-like modifier-activating enzyme 6	polymer	1016	114475.9	1	UniProt (A0AVT1)	Homo sapiens (human)	Ubiquitin-activating enzyme 6,Monocyte protein 4,MOP-4,Ubiquitin-activating enzyme E1-like protein 2,E1-L2
2	B (B)	Ubiquitin-conjugating enzyme E2 Z	polymer	262	29965.0	1	UniProt (Q9H832)	Homo sapiens (human)	E2 ubiquitin-conjugating enzyme Z,Uba6-specific E2 conjugating enzyme 1,Use1,Ubiquitin carrier protein Z,Ubiquitin-protein ligase Z
3	C, D (C, D)	Ubiquitin D	polymer	165	18476.5	2	UniProt (A0A1U9X8S9)	Homo sapiens (human)	Diubiquitin,Ubiquitin-like protein FAT10
4	E (A)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP) PubChem (6083)
5	F (A)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	1	Chemie (IHP)
6	G (A)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)

Sequence modifications

B: 93 - 354 (UniProt: Q9H832)

PDB	External Database	Details
Ser 100	Cys 100	engineered mutation
Arg 130	Lys 130	engineered mutation
Arg 166	Lys 166	engineered mutation
Lys 182	Tyr 182	engineered mutation
Arg 186	Lys 186	engineered mutation
Ser 261	Cys 261	engineered mutation
Ser 263	Cys 263	engineered mutation

C, D: 1 - 165 (UniProt: A0A1U9X8S9)

PDB	External Database	Details
Thr 7	Cys 7	engineered mutation
Thr 9	Cys 9	engineered mutation
Leu 134	Cys 134	engineered mutation
Ser 162	Cys 162	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	181393.8
Non-Polymers*	Number of molecules	3
	Total formula weight	1183.2
All*	Total formula weight	182577.0

*Water molecules are not included.