9YK5
100K X-ray structure of mixed metal D132N Bacillus halodurans RNase H1 complex with RNA/DNA duplex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ribonuclease H | polymer | 139 | 16003.1 | 1 | UniProt (Q9KEI9) | Halalkalibacterium halodurans | RNase H |
| 2 | B (B) | RNA (5'-R(*UP*CP*GP*AP*CP*A)-3') | polymer | 6 | 1875.2 | 1 | Halalkalibacterium halodurans | ||
| 3 | C (C) | DNA (5'-D(*AP*TP*GP*TP*CP*G)-3') | polymer | 6 | 1824.2 | 1 | Halalkalibacterium halodurans | ||
| 4 | D (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) PubChem (27854) | |||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 6 | F (C) | PHOSPHITE ION | non-polymer | 79.0 | 1 | Chemie (PO3) | |||
| 7 | G, H, I (A, B, C) | water | water | 18.0 | 31 | Chemie (HOH) |
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Ser 58 | - | expression tag |
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 19702.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 158.2 | |
| All* | Total formula weight | 19860.7 |
