9YHX

DNA ligase 1 E346A/E592A in complex with nick containing 3'-8oxodG:C captured at pre-catalytic stage

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA ligase 1	polymer	668	74213.5	1	UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679)	Homo sapiens (human)	DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1
2	B (B)	DNA (5'-D(P*GP*CP*TP*GP*AP*TP*GP*CP*GP*TP*(8OG))-3')	polymer	11	3421.2	1		synthetic construct
3	C (C)	DNA (5'-D(P*(AMP)P*GP*TP*CP*GP*GP*AP*C)-3')	polymer	8	2467.6	1		synthetic construct
4	D (D)	DNA (5'-D(P*GP*TP*CP*CP*GP*AP*CP*CP*AP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')	polymer	18	5446.5	1		synthetic construct
5	E, F, G, H (A, B, C, D)	water	water		18.0	19	Chemie (HOH)

Sequence modifications

A: 262 - 923 (UniProt: P18858)

PDB	External Database	Details
Ala 346	Glu 346	engineered mutation
Ala 592	Glu 592	engineered mutation
Ala 924	-	expression tag
Ala 925	-	expression tag
Ala 926	-	expression tag
Leu 927	-	expression tag
Glu 928	-	expression tag
His 929	-	expression tag
His 930	-	expression tag
His 931	-	expression tag
His 932	-	expression tag
His 933	-	expression tag
His 934	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	85548.9
All*	Total formula weight	85548.9

*Water molecules are not included.