9YGZ
Cryo-EM structure of active mutant human green cone opsin (E129Q) in complex with chimeric G protein (miniGist)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Medium-wave-sensitive opsin 1 | polymer | 365 | 40784.4 | 1 | UniProt (P04001) Pfam (PF00001) | Homo sapiens (human) | Green cone photoreceptor pigment,Green-sensitive opsin,GOP |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 357 | 38878.4 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (H) | scFv16 | polymer | 268 | 28674.9 | 1 | Mus musculus | ||
| 5 | E (A) | Genome polyprotein,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas | polymer | 509 | 57380.7 | 1 | UniProt (W8GG88) UniProt (Q5JWF2) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas |
| 6 | F (R) | RETINAL | non-polymer | 284.4 | 1 | Chemie (RET) PubChem (638015) PubChem (5381765) |
Sequence modifications
R: 1 - 355 (UniProt: P04001)
B: 1 - 340 (UniProt: P62873)
A: -240 - -3 (UniProt: W8GG88)
A: 170 - 339 (UniProt: Q5JWF2)
| PDB | External Database | Details |
|---|---|---|
| Gln 129 | Glu 129 | engineered mutation |
| Thr 356 | - | expression tag |
| Glu 357 | - | expression tag |
| Thr 358 | - | expression tag |
| Ser 359 | - | expression tag |
| Gln 360 | - | expression tag |
| Val 361 | - | expression tag |
| Ala 362 | - | expression tag |
| Pro 363 | - | expression tag |
| Ala 364 | - | expression tag |
| Unk 365 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Ser 346 | - | expression tag |
| Ser 347 | - | expression tag |
| Gly 348 | - | expression tag |
| Thr 349 | - | expression tag |
| Glu 350 | - | expression tag |
| Thr 351 | - | expression tag |
| Ser 352 | - | expression tag |
| Gln 353 | - | expression tag |
| Val 354 | - | expression tag |
| Ala 355 | - | expression tag |
| Pro 356 | - | expression tag |
| Ala 357 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -262 | - | expression tag |
| Leu -261 | - | expression tag |
| Gln -260 | - | expression tag |
| Asn -259 | - | expression tag |
| Glu -258 | - | expression tag |
| Leu -257 | - | expression tag |
| Ala -256 | - | expression tag |
| Leu -255 | - | expression tag |
| Lys -254 | - | expression tag |
| Leu -253 | - | expression tag |
| Ala -252 | - | expression tag |
| Gly -251 | - | expression tag |
| Leu -250 | - | expression tag |
| Asp -249 | - | expression tag |
| Ile -248 | - | expression tag |
| Asn -247 | - | expression tag |
| Lys -246 | - | expression tag |
| Thr -245 | - | expression tag |
| Gly -244 | - | expression tag |
| Gly -243 | - | expression tag |
| Ser -242 | - | expression tag |
| Gly -241 | - | expression tag |
| Phe -177 | Leu 2420 | conflict |
| Gly -176 | Thr 2421 | conflict |
| Leu -173 | Val 2424 | conflict |
| Ala -169 | Ser 2428 | conflict |
| Tyr -38 | Thr 2559 | conflict |
| Gly -2 | - | linker |
| Ser -1 | - | linker |
| Gly 0 | - | linker |
| Ser 1 | - | linker |
| Gly 2 | - | linker |
| Cys 3 | - | linker |
| Thr 4 | - | linker |
| Leu 5 | - | linker |
| Ser 6 | - | linker |
| Ala 7 | - | linker |
| Glu 8 | - | linker |
| Asp 9 | - | linker |
| Lys 10 | - | linker |
| Ala 11 | - | linker |
| Ala 12 | - | linker |
| Val 13 | - | linker |
| Glu 14 | - | linker |
| Arg 15 | - | linker |
| Ser 16 | - | linker |
| Lys 17 | - | linker |
| Met 18 | - | linker |
| Ile 19 | - | linker |
| Glu 20 | - | linker |
| Lys 21 | - | linker |
| Gln 22 | - | linker |
| Leu 23 | - | linker |
| Gln 24 | - | linker |
| Lys 25 | - | linker |
| Asp 26 | - | linker |
| Lys 27 | - | linker |
| Gln 28 | - | linker |
| Val 29 | - | linker |
| Tyr 30 | - | linker |
| Arg 31 | - | linker |
| Ala 32 | - | linker |
| Thr 33 | - | linker |
| His 34 | - | linker |
| Arg 35 | - | linker |
| Leu 36 | - | linker |
| Leu 37 | - | linker |
| Leu 38 | - | linker |
| Leu 39 | - | linker |
| Gly 40 | - | linker |
| Ala 41 | - | linker |
| Asp 42 | - | linker |
| Asn 43 | - | linker |
| Ser 44 | - | linker |
| Gly 45 | - | linker |
| Lys 46 | - | linker |
| Ser 47 | - | linker |
| Thr 48 | - | linker |
| Ile 49 | - | linker |
| Val 50 | - | linker |
| Lys 51 | - | linker |
| Gln 52 | - | linker |
| Met 157 | - | linker |
| Arg 158 | - | linker |
| Ile 159 | - | linker |
| Leu 160 | - | linker |
| His 161 | - | linker |
| Gly 162 | - | linker |
| Gly 163 | - | linker |
| Ser 164 | - | linker |
| Gly 165 | - | linker |
| Gly 166 | - | linker |
| Ser 167 | - | linker |
| Gly 168 | - | linker |
| Gly 169 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asp 215 | Ala 892 | conflict |
| Asp 218 | Ser 895 | conflict |
| - | Met 898 | deletion |
| - | Val 899 | deletion |
| - | Ile 900 | deletion |
| - | Arg 901 | deletion |
| - | Glu 902 | deletion |
| - | Asp 903 | deletion |
| - | Asn 904 | deletion |
| - | Gln 905 | deletion |
| - | Thr 906 | deletion |
| - | Asn 907 | deletion |
| Asp 228 | Leu 915 | conflict |
| Ala 328 | Ile 1015 | conflict |
| Ile 331 | Val 1018 | conflict |
| Val 335 | Cys 1022 | conflict |
| Thr 336 | Arg 1023 | conflict |
| Ile 340 | - | expression tag |
| Leu 341 | - | expression tag |
| Glu 342 | - | expression tag |
| Asp 343 | - | expression tag |
| Leu 344 | - | expression tag |
| Lys 345 | - | expression tag |
| Ser 346 | - | expression tag |
| Cys 347 | - | expression tag |
| Gly 348 | - | expression tag |
| Leu 349 | - | expression tag |
| Phe 350 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 173579.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 284.4 | |
| All* | Total formula weight | 173864.0 |
