9YDA

Cryo-EM structure of active human green cone opsin in complex with chimeric G protein (miniGist)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Medium-wave-sensitive opsin 1	polymer	364	40700.3	1	UniProt (P04001)	Homo sapiens (human)	Green cone photoreceptor pigment,Green-sensitive opsin,GOP
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	38878.4	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (H)	scFv16	polymer	259	27784.9	1		Mus musculus
5	E (A)	Genome polyprotein,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	509	57380.7	1		Homo sapiens (human)
6	F (R)	RETINAL	non-polymer		284.4	1	Chemie (RET)

Sequence modifications

R: 1 - 355 (UniProt: P04001)

PDB	External Database	Details
Thr 356	-	expression tag
Glu 357	-	expression tag
Thr 358	-	expression tag
Ser 359	-	expression tag
Gln 360	-	expression tag
Val 361	-	expression tag
Ala 362	-	expression tag
Pro 363	-	expression tag
Ala 364	-	expression tag

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Ser 346	-	expression tag
Ser 347	-	expression tag
Gly 348	-	expression tag
Thr 349	-	expression tag
Glu 350	-	expression tag
Thr 351	-	expression tag
Ser 352	-	expression tag
Gln 353	-	expression tag
Val 354	-	expression tag
Ala 355	-	expression tag
Pro 356	-	expression tag
Ala 357	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	172605.4
Non-Polymers*	Number of molecules	1
	Total formula weight	284.4
All*	Total formula weight	172889.9

*Water molecules are not included.