9YCX

Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the Non-Canonical Orientation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	344	37671.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (L)	Tachykinin-1	polymer	13	1514.9	1	UniProt (Q8I6S3)	Octopus vulgaris (common octopus)	OctTK-I,Tachykinin I
4	D (R)	Neurotensin receptor type 1	polymer	445	49531.0	1	UniProt (P30989) Pfam (PF00001)	Homo sapiens (human)	NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH
5	E (A)	Guanine nucleotide-binding protein G(i) subunit alpha-3	polymer	354	40584.2	1	UniProt (P08754) Pfam (PF00503)	Homo sapiens (human)	G(i) alpha-3
6	F (S)	scFv16	polymer	259	27784.9	1		Mus musculus

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 20 - 418 (UniProt: P30989)

PDB	External Database	Details
Asp -19	-	expression tag
Tyr -18	-	expression tag
Lys -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Ala -12	-	expression tag
Met -11	-	expression tag
Gly -10	-	expression tag
Gln -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Asn -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Phe -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Ala 1	-	expression tag
Pro 2	-	expression tag
Asn 3	-	expression tag
Arg 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
Ala 7	-	expression tag
Pro 8	-	expression tag
Asp 9	-	expression tag
His 10	-	expression tag
Asp 11	-	expression tag
Val 12	-	expression tag
Glu 13	-	expression tag
Asn 14	-	expression tag
Leu 15	-	expression tag
Tyr 16	-	expression tag
Phe 17	-	expression tag
Gln 18	-	expression tag
Gly 19	-	expression tag
Leu 85	Ala 85	conflict
Leu 419	-	expression tag
Glu 420	-	expression tag
Val 421	-	expression tag
Leu 422	-	expression tag
Phe 423	-	expression tag
Gln 424	-	expression tag
Gly 425	-	expression tag

A: 1 - 354 (UniProt: P08754)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	164947.1
All*	Total formula weight	164947.1

*Water molecules are not included.