9Y90
Cryo-EM structure of Broadly neutralizing antibody VRC48.02 in complex with HIV-1 Env BG505 DS-SOSIP trimer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (F, A, E) | VRC48.02 Heavy | polymer | 124 | 14143.8 | 3 | Homo sapiens | ||
| 2 | B, F, J (I, B, G) | VRC48.02 Light | polymer | 106 | 11700.2 | 3 | Homo sapiens | ||
| 3 | C, G, K (J, C, H) | BG505 SOSIP.664 gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S5) | Human immunodeficiency virus 1 | |
| 4 | D, H, L (K, D, L) | HIV-1 BG505 DS-SOSIP gp120 | polymer | 481 | 54086.3 | 3 | UniProt (Q2N0S6) | Human immunodeficiency virus 1 | |
| 5 | DA, EA, FA, IA, JA... (d, e, f, i, j...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 18 | In PDB GlyTouCan (G42666HT) | |||
| 6 | AA, LA, P (a, l, P) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 3 | In PDB GlyTouCan (G81315DD) | |||
| 7 | BA, HA, MA, Q, SA... (b, h, m, Q, s...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 6 | In PDB GlyTouCan (G15407YE) | |||
| 8 | CA, GA, NA, R, RA... (c, g, n, R, r...) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 6 | In PDB GlyTouCan (G22768VO) | |||
| 9 | AB, BB, CB, DB, EB... (K, C, D, H, L...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 33 | Chemie (NAG) PubChem (24139) |
Sequence modifications
J, C, H: 512 - 664 (UniProt: Q2N0S5)
K, D, L: 31 - 508 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 201 | Ile 200 | engineered mutation |
| Asn 332 | Thr 330 | engineered mutation |
| Cys 433 | Ala 430 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 291230.6 | |
| Branched | Number of molecules | 33 |
| Total formula weight | 18869.5 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 7299.9 | |
| All* | Total formula weight | 317399.9 |
