9Y6B

CRYSTAL STRUCTURE OF A149T VARIANT OF SERINE HYDROXYMETHYLTRANSFERASE 8 FROM SOYBEAN CULTIVAR ESSEX IN COMPLEX WITH PLP-GLYCINE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Serine hydroxymethyltransferase	polymer	496	54723.0	5	UniProt (K4FZF8)	Glycine max (soybean)
2	F, G, H, I, J (A, B, C, D, E)	N-GLYCINE-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE]	non-polymer		306.2	5	Chemie (PLG) PubChem (445062)
3	K, L, M, N, O (A, B, C, D, E)	water	water		18.0	628	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 471 (UniProt: K4FZF8)

PDB	External Database	Details
Met -24	-	initiating methionine
Gly -23	-	expression tag
Met -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Thr 149	Ala 149	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	273614.8
Non-Polymers*	Number of molecules	5
	Total formula weight	1531.0
All*	Total formula weight	275145.9

*Water molecules are not included.