9Y61

Cryo-EM structure of ternary complex NSD2-PWWP1:CRBN:DDB1 in complex with NSD2-LDD, an LDD degrader

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone-lysine N-methyltransferase NSD2	polymer	174	19818.7	1	UniProt (O96028)	Homo sapiens (human)	Multiple myeloma SET domain-containing protein,MMSET,Nuclear SET domain-containing protein 2,Protein trithorax-5,Wolf-Hirschhorn syndrome candidate 1 protein
2	B (B)	Protein cereblon	polymer	467	53582.0	1	UniProt (Q96SW2)	Homo sapiens (human)
3	C (A)	1-(5-{9-[(4-{2-[(2S,3R)-2-(3,5-dichloro-4-fluorophenyl)-5-oxomorpholin-3-yl]ethyl}piperazin-1-yl)methyl]-3-azaspiro[5.5]undecane-3-carbonyl}-2-methylphenyl)-1,3-diazinane-2,4-dione	non-polymer		771.7	1	Chemie (A1CSQ)
4	D (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN) PubChem (32051)

Sequence modifications

A: 202 - 368 (UniProt: O96028)

PDB	External Database	Details
Met 201	-	initiating methionine
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag

B: 1 - 442 (UniProt: Q96SW2)

PDB	External Database	Details
Met -24	-	expression tag
Ser -23	-	expression tag
Tyr -22	-	expression tag
Tyr -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73400.7
Non-Polymers*	Number of molecules	2
	Total formula weight	837.2
All*	Total formula weight	74237.8

*Water molecules are not included.