9Y5U

eIF2B lacking the latch helix bound to ISRACT-02 (Inactive state)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Translation initiation factor eIF-2B subunit epsilon	polymer	721	80452.6	2	UniProt (Q13144)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit epsilon
2	C, D (C, D)	Translation initiation factor eIF2B subunit beta	polymer	355	39438.9	2	UniProt (P49770)	Homo sapiens (human)	S20I15,S20III15,eIF2B GDP-GTP exchange factor subunit beta
3	E, F (E, F)	Translation initiation factor eIF-2B subunit delta	polymer	523	57640.2	2	UniProt (Q9UI10)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit delta
4	G, H (G, H)	Translation initiation factor eIF-2B subunit alpha	polymer	305	33754.1	2	UniProt (Q14232)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit alpha
5	I, J (I, J)	Translation initiation factor eIF-2B subunit gamma	polymer	452	50304.2	2	UniProt (Q9NR50)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit gamma
6	K (C)	3-(4-chlorophenoxy)-N-{(1r,4r)-4-[2-(4-chlorophenoxy)acetamido]cyclohexyl}-N-[(pyridin-4-yl)methyl]propanamide	non-polymer		556.5	1	Chemie (A1CSR)
7	L, O (E, F)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
8	M, N (E, F)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL) PubChem (312)

Sequence modifications

A, B: 1 - 721 (UniProt: Q13144)

PDB	External Database	Details
Val 587	Ile 587	conflict

C, D: 2 - 351 (UniProt: P49770)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag
Gly 113	Ser 100	linker
Ser 114	Asp 101	linker
Gly 115	Glu 102	linker
-	Asp 104	deletion
-	Gln 105	deletion
-	Gln 106	deletion
-	Glu 107	deletion
-	Ser 108	deletion
-	Leu 109	deletion
-	His 110	deletion
-	Lys 111	deletion
-	Leu 112	deletion
-	Leu 113	deletion
-	Thr 114	deletion
-	Ser 115	deletion
-	Gly 116	deletion

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	523180.1
Non-Polymers*	Number of molecules	5
	Total formula weight	758.2
All*	Total formula weight	523938.3

*Water molecules are not included.