9Y3T

Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta subunit (active-like-state)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Translation initiation factor eIF-2B subunit epsilon	polymer	721	80452.6	2	UniProt (Q13144) Pfam (PF02020)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit epsilon
2	C, D (C, D)	Translation initiation factor eIF2B subunit beta	polymer	355	39438.9	2	UniProt (P49770) Pfam (PF01008)	Homo sapiens (human)	S20I15,S20III15,eIF2B GDP-GTP exchange factor subunit beta
3	E, F (E, F)	Translation initiation factor eIF-2B subunit delta	polymer	523	57640.2	2	UniProt (Q9UI10) Pfam (PF01008)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit delta
4	G (G)	Translation initiation factor eIF2B subunit alpha	polymer	306	33841.2	1	UniProt (Q14232) Pfam (PF01008)	Homo sapiens (human)	eIF2B GDP-GTP exchange factor subunit alpha
5	H (H)	Translation initiation factor eIF-2B subunit alpha	polymer	305	33754.1	1	UniProt (Q14232) Pfam (PF01008)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit alpha
6	I, J (I, J)	Translation initiation factor eIF-2B subunit gamma	polymer	452	50304.2	2	UniProt (Q9NR50) Pfam (PF00483) Pfam (PF25084)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit gamma
7	K, L, O, R (A, B, E, F)	ZINC ION	non-polymer		65.4	4	Chemie (ZN) PubChem (32051)
8	M, N (C, D)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4) PubChem (1061)
9	P, Q (E, F)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL) PubChem (312)

Sequence modifications

A, B: 1 - 721 (UniProt: Q13144)

PDB	External Database	Details
Val 587	Ile 587	conflict

C, D: 2 - 351 (UniProt: P49770)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag
Gly 100	Ser 100	conflict
Ser 101	Asp 101	conflict
Gly 102	Glu 102	conflict
-	Asp 104	deletion
-	Gln 105	deletion
-	Gln 106	deletion
-	Glu 107	deletion
-	Ser 108	deletion
-	Leu 109	deletion
-	His 110	deletion
-	Lys 111	deletion
-	Leu 112	deletion
-	Leu 113	deletion
-	Thr 114	deletion
-	Ser 115	deletion
-	Gly 116	deletion

G: 2 - 305 (UniProt: Q14232)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	523267.2
Non-Polymers*	Number of molecules	8
	Total formula weight	522.5
All*	Total formula weight	523789.7

*Water molecules are not included.