9Y1N
Cryo EM Structure of Full lengthmGluR8 Bound to Agonist L-AP4 and PAM VU6005649 in complex with G proteins
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Metabotropic glutamate receptor 8 | polymer | 841 | 94195.5 | 2 | UniProt (O00222) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) | Homo sapiens (human) | mGluR8 |
| 2 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 3 | D (D) | scFv-14 | polymer | 259 | 27784.9 | 1 | Mus musculus (Mouse) | ||
| 4 | E (E) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 5 | F (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 357 | 39286.9 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 6 | G, I (A, B) | (2S)-2-amino-4-phosphonobutanoic acid | non-polymer | 183.1 | 2 | Chemie (E7P) PubChem (179394) | |||
| 7 | H (A) | (3P,8S)-3-(2,3-difluoro-4-methoxyphenyl)-2,5-dimethyl-7-(trifluoromethyl)pyrazolo[1,5-a]pyrimidine | non-polymer | 357.3 | 1 | Chemie (A1BWG) |
Sequence modifications
A, B: 34 - 848 (UniProt: O00222)
G: 7 - 345 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Met 8 | - | initiating methionine |
| Lys 9 | - | expression tag |
| Thr 10 | - | expression tag |
| Ile 11 | - | expression tag |
| Ile 12 | - | expression tag |
| Ala 13 | - | expression tag |
| Leu 14 | - | expression tag |
| Ser 15 | - | expression tag |
| Tyr 16 | - | expression tag |
| Ile 17 | - | expression tag |
| Phe 18 | - | expression tag |
| Cys 19 | - | expression tag |
| Leu 20 | - | expression tag |
| Val 21 | - | expression tag |
| Phe 22 | - | expression tag |
| Ala 23 | - | expression tag |
| Asp 24 | - | expression tag |
| Tyr 25 | - | expression tag |
| Lys 26 | - | expression tag |
| Asp 27 | - | expression tag |
| Asp 28 | - | expression tag |
| Asp 29 | - | expression tag |
| Asp 30 | - | expression tag |
| Ala 31 | - | expression tag |
| Ala 32 | - | expression tag |
| Ala 33 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Glu -4 | - | expression tag |
| Val -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Gln 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Gly 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Ser 5 | - | expression tag |
| Gly 6 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 303738.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 723.5 | |
| All* | Total formula weight | 304462.4 |
