9Y03

Cryo-EM structure of human VCP/p97-R89W mutant bound to ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Transitional endoplasmic reticulum ATPase	polymer	822	91274.8	6	UniProt (P55072) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862)	Homo sapiens (human)	TER ATPase,15S Mg(2+)-ATPase p97 subunit,Valosin-containing protein,VCP
2	G, H, I, J, K... (A, B, C, D, E...)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	12	Chemie (ADP)

Sequence modifications

A, B, C, D, E, F: 1 - 806 (UniProt: P55072)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Trp 89	Arg 89	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	547648.8
Non-Polymers*	Number of molecules	12
	Total formula weight	5126.4
All*	Total formula weight	552775.2

*Water molecules are not included.