9XVI

Arabidopsis ISA2-ISA1-ISA1-ISA2 heterotetramer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Isoamylase 1, chloroplastic	polymer	764	87301.9	2	UniProt (O04196)	Arabidopsis thaliana (thale cress)	AtISA1,Protein REDUCED GRAVITROPIC 3
2	B, D (C, D)	Isoamylase 2, chloroplastic	polymer	870	97595.5	2	UniProt (Q8L735)	Arabidopsis thaliana (thale cress)	AtISA2,Protein DEBRANCHING ENZYME 1

Sequence modifications

A, B: 1 - 740 (UniProt: O04196)

PDB	External Database	Details
Met -23	-	initiating methionine
Ala -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

C, D: -16 - 812 (UniProt: Q8L735)

PDB	External Database	Details
Met -57	-	initiating methionine
Asp -56	-	expression tag
Tyr -55	-	expression tag
Lys -54	-	expression tag
Asp -53	-	expression tag
Asp -52	-	expression tag
Asp -51	-	expression tag
Asp -50	-	expression tag
Lys -49	-	expression tag
Trp -48	-	expression tag
Ser -47	-	expression tag
His -46	-	expression tag
Pro -45	-	expression tag
Gln -44	-	expression tag
Phe -43	-	expression tag
Glu -42	-	expression tag
Lys -41	-	expression tag
Gly -40	-	expression tag
Gly -39	-	expression tag
Gly -38	-	expression tag
Gly -37	-	expression tag
Ser -36	-	expression tag
Gly -35	-	expression tag
Gly -34	-	expression tag
Ser -33	-	expression tag
Ala -32	-	expression tag
Trp -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
Pro -28	-	expression tag
Gln -27	-	expression tag
Phe -26	-	expression tag
Glu -25	-	expression tag
Lys -24	-	expression tag
Glu -23	-	expression tag
Phe -22	-	expression tag
Lys -21	-	expression tag
Gly -20	-	expression tag
Leu -19	-	expression tag
Val -18	-	expression tag
Asp -17	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	369794.8
All*	Total formula weight	369794.8

*Water molecules are not included.