9XOU

CryoEM structure of LacY with Trimbody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	H3-PrAC-5350A,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase	polymer	332	36127.9	3	UniProt (Q9WXS1) Pfam (PF01081)	synthetic construct
2	D, E, F (D, E, F)	LacY-nanobody-TAIL	polymer	256	28210.1	3		synthetic construct
3	G, H, I (G, H, I)	Lactose permease	polymer	437	49236.6	3	UniProt (P02920) Pfam (PF01306)	Escherichia coli K-12	Lactose-proton symport

Sequence modifications

A, B, C: 1 - 117 (PDB: 9XOU)
A, B, C: 118 - 321 (UniProt: Q9WXS1)

PDB	External Database	Details
Ile 141	Lys 25	conflict
Val 145	Leu 29	conflict
Ala 149	Glu 33	conflict
Ala 170	Glu 54	conflict
Val 173	Phe 57	conflict
Ala 322	-	expression tag
Ala 323	-	expression tag
Ala 324	-	expression tag
Leu 325	-	expression tag
Glu 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag

G, H, I: 1 - 417 (UniProt: P02920)

PDB	External Database	Details
Trp 46	Gly 46	conflict
Ser 117	Cys 117	conflict
Ala 148	Cys 148	conflict
Val 154	Cys 154	conflict
Met 176	Cys 176	conflict
Ser 234	Cys 234	conflict
Trp 262	Gly 262	conflict
Ser 333	Cys 333	conflict
Ala 353	Cys 353	conflict
Ala 355	Cys 355	conflict
Trp 418	-	expression tag
Ser 419	-	expression tag
His 420	-	expression tag
Pro 421	-	expression tag
Gln 422	-	expression tag
Phe 423	-	expression tag
Glu 424	-	expression tag
Lys 425	-	expression tag
Gly 426	-	expression tag
Ser 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	340723.9
All*	Total formula weight	340723.9

*Water molecules are not included.