9XDQ

Cryo-EM structure of BMS986187 bound DOR-Gi complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Soluble cytochrome b562,Delta-type opioid receptor,Soluble cytochrome b562,Delta-type opioid receptor,Oplophorus-luciferin 2-monooxygenase catalytic subunit	polymer	595	66442.4	1	UniProt (P0ABE7) UniProt (P41143)	Escherichia coli	Cytochrome b-562,D-OR-1,DOR-1
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	384	41729.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (D)	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40502.9	1	UniProt (P04899)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
5	E (A)	3,3,6,6-tetramethyl-9-[4-[(2-methylphenyl)methoxy]phenyl]-4,5,7,9-tetrahydro-2~{H}-xanthene-1,8-dione	non-polymer		470.6	1	Chemie (A1D6B) PubChem (17379334)

Sequence modifications

A: -66 - 40 (UniProt: P0ABE7)

PDB	External Database	Details
Met -89	-	initiating methionine
Lys -88	-	expression tag
Thr -87	-	expression tag
Ile -86	-	expression tag
Ile -85	-	expression tag
Ala -84	-	expression tag
Leu -83	-	expression tag
Ser -82	-	expression tag
Tyr -81	-	expression tag
Ile -80	-	expression tag
Phe -79	-	expression tag
Cys -78	-	expression tag
Leu -77	-	expression tag
Val -76	-	expression tag
Phe -75	-	expression tag
Ala -74	-	expression tag
Asp -73	-	expression tag
Tyr -72	-	expression tag
Lys -71	-	expression tag
Asp -70	-	expression tag
Asp -69	-	expression tag
Asp -68	-	expression tag
Asp -67	-	expression tag
Trp -59	Met 29	conflict
Ile 36	His 124	conflict
Leu 40	Arg 128	conflict

A: 41 - 347 (UniProt: P41143)
A: 348 - 505 (PDB: 9XDQ)
B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ser 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

D: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	conflict
Ala 204	Gly 204	conflict
Ala 246	Glu 246	conflict
Ser 327	Ala 327	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156536.0
Non-Polymers*	Number of molecules	1
	Total formula weight	470.6
All*	Total formula weight	157006.6

*Water molecules are not included.