9X25

Target analog-bound type III-B Cmr complex of Archaeoglobus fulgidus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	crRNA	polymer	39	12604.5	1		Pyrococcus furiosus DSM 3638
2	B (B)	GGDEF domain-containing protein	polymer	1024	117383.2	1	UniProt (O28412) Pfam (PF12469) Pfam (PF22335)	Archaeoglobus fulgidus DSM 4304
3	C (C)	Type III-B CRISPR module-associated protein Cmr3	polymer	341	39043.3	1	UniProt (O28413) Pfam (PF09700)	Archaeoglobus fulgidus DSM 4304
4	D, E, F (D, E, F)	CRISPR type III-associated protein domain-containing protein	polymer	355	39677.6	3	UniProt (O28416) Pfam (PF03787)	Archaeoglobus fulgidus DSM 4304
5	G, H (G, H)	CRISPR system Cmr subunit Cmr5	polymer	155	17407.7	2	UniProt (O28417) Pfam (PF09701)	Archaeoglobus fulgidus DSM 4304	CRISPR type III-B/RAMP module-associated protein Cmr5
6	I (I)	CRISPR type III-associated protein domain-containing protein	polymer	349	40298.8	1	UniProt (O28418) Pfam (PF03787)	Archaeoglobus fulgidus DSM 4304
7	J (K)	Target analog DNA	polymer	31	9451.1	1		synthetic construct

Sequence modifications

B: 1 - 1004 (UniProt: O28412)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

I: 1 - 343 (UniProt: O28418)

PDB	External Database	Details
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	372629.0
All*	Total formula weight	372629.0

*Water molecules are not included.