9WXM

Cryo-EM structure of the full-length GPR15L bound GPR15-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	Protein GPR15LG	polymer	58	6591.9	1	UniProt (Q6UWK7) Pfam (PF15854)	Homo sapiens (human)	Antimicrobial peptide with 57 amino acid residues,AP-57,Antimicrobial peptide-57,Colon-derived SUSD2 binding factor,CSBF,Protein GPR15 ligand,Protein GPR15L,Secreted protein C10orf99
2	B (R)	G-protein coupled receptor 15	polymer	379	42638.7	1	UniProt (P49685) Pfam (PF00001)	Homo sapiens (human)	Brother of Bonzo,BoB
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-3	polymer	354	40617.2	1	UniProt (P08754) Pfam (PF00503)	Homo sapiens (human)	G(i) alpha-3
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (D)	scFv16	polymer	267	28668.9	1		Mus musculus

Sequence modifications

L: 1 - 57 (UniProt: Q6UWK7)

PDB	External Database	Details
Gly 0	-	expression tag

R: 1 - 330 (UniProt: P49685)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Pro 0	-	expression tag
Glu 331	-	expression tag
Phe 332	-	expression tag
Leu 333	-	expression tag
Glu 334	-	expression tag
Val 335	-	expression tag
Leu 336	-	expression tag
Phe 337	-	expression tag
Gln 338	-	expression tag
Gly 339	-	expression tag
Pro 340	-	expression tag
Trp 341	-	expression tag
Ser 342	-	expression tag
His 343	-	expression tag
Pro 344	-	expression tag
Gln 345	-	expression tag
Phe 346	-	expression tag
Glu 347	-	expression tag
Lys 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Ser 352	-	expression tag
Gly 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Ser 356	-	expression tag
Gly 357	-	expression tag
Gly 358	-	expression tag
Ser 359	-	expression tag
Ala 360	-	expression tag
Trp 361	-	expression tag
Ser 362	-	expression tag
His 363	-	expression tag
Pro 364	-	expression tag
Gln 365	-	expression tag
Phe 366	-	expression tag
Glu 367	-	expression tag
Lys 368	-	expression tag
Asp 369	-	expression tag
Tyr 370	-	expression tag
Lys 371	-	expression tag
Asp 372	-	expression tag
Asp 373	-	expression tag
Asp 374	-	expression tag
Asp 375	-	expression tag
Lys 376	-	expression tag

A: 1 - 354 (UniProt: P08754)

PDB	External Database	Details
Cys 47	Ser 47	conflict
Thr 202	Gly 202	conflict
Ala 203	Gly 203	conflict
Ala 245	Glu 245	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	165122.3
All*	Total formula weight	165122.3

*Water molecules are not included.