9WXB

Cryo-EM structure of reduced form of formatedehydrogenase from Rhodobacter aestuarii (RaFDH) with NADH

Replaces:  9KTLReplaces:  9KTR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (G, H)	Formate dehydrogenase delta subunit	polymer	70	7180.2	2	UniProt (A0A1N7KD80)	Rhodobacter aestuarii
2	B, G (E, F)	Formate dehydrogenase gamma subunit	polymer	179	19331.0	2	UniProt (A0A1N7KDI2)	Rhodobacter aestuarii
3	C, F (B, A)	Formate dehydrogenase alpha subunit	polymer	957	103751.0	2	UniProt (A0A1N7KDD5)	Rhodobacter aestuarii
4	E, H (C, D)	Formate dehydrogenase beta subunit	polymer	501	52942.6	2	UniProt (A0A1N7KDE1)	Rhodobacter aestuarii
5	CA, I, M, X (F, E, B, A)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	4	Chemie (FES)
6	J, K, U, V (B, A)	2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE	non-polymer		740.6	4	Chemie (MGD)
7	L, W (B, A)	MOLYBDENUM(VI) ION	non-polymer		95.9	2	Chemie (6MO)
8	AA, BA, FA, N, O... (A, D, B, C)	IRON/SULFUR CLUSTER	non-polymer		351.6	10	Chemie (SF4)
9	DA, R (D, C)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
10	EA, S (D, C)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN) PubChem (643976)

Sequence modifications

E, F: 1 - 150 (UniProt: A0A1N7KDI2)

PDB	External Database	Details
Ser -28	-	expression tag
Arg -27	-	expression tag
Ala -26	-	expression tag
Trp -25	-	expression tag
Arg -24	-	expression tag
His -23	-	expression tag
Pro -22	-	expression tag
Gln -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Gly -11	-	expression tag
Met -10	-	expression tag
Arg -9	-	expression tag
Asp -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	366409.5
Non-Polymers*	Number of molecules	24
	Total formula weight	9613.3
All*	Total formula weight	376022.8

*Water molecules are not included.