9WST
Cryo-EM structure of DAMGO-muOR-Gz-scFv16 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Engineered guanine nucleotide-binding protein G(z) subunit alpha | polymer | 355 | 40699.5 | 1 | Homo sapiens | ||
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 371 | 40039.6 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (R) | Mu-type opioid receptor,LgBiT | polymer | 575 | 64018.8 | 1 | UniProt (P35372) Pfam (PF00001) | Homo sapiens (human) | M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP |
| 5 | E (P) | DAMGO | polymer | 5 | 513.6 | 1 | BIRD (PRD_002308) | synthetic construct | |
| 6 | F (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: 5 - 398 (UniProt: P35372)
R: 399 - 570 (PDB: 9WST)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Val 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Trp 359 | - | expression tag |
| Arg 360 | - | expression tag |
| Leu 361 | - | expression tag |
| Phe 362 | - | expression tag |
| Lys 363 | - | expression tag |
| Lys 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Ser 366 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Asp 1 | - | expression tag |
| Asp 2 | - | expression tag |
| Lys 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Gly 6 | Thr 8 | conflict |
| Ile 8 | Ala 10 | conflict |
| Asp 10 | Asn 12 | conflict |
| Ser 12 | Thr 14 | conflict |
| Pro 14 | Ala 16 | conflict |
| Pro 17 | Tyr 19 | conflict |
| Ala 18 | Ser 20 | conflict |
| Leu 30 | Val 32 | conflict |
| Val 35 | Leu 37 | conflict |
| Gln 39 | Leu 41 | conflict |
| Gly 49 | Asp 51 | conflict |
| Ser 53 | Arg 55 | conflict |
| His 54 | Asp 56 | conflict |
| Gln 59 | Pro 61 | conflict |
| Val 66 | Ile 68 | conflict |
| Asn 137 | Thr 139 | conflict |
| Val 187 | Ile 189 | conflict |
| Ile 306 | Val 308 | conflict |
| Thr 357 | Asn 359 | conflict |
| Ala 364 | Thr 366 | conflict |
| Glu 372 | Asp 374 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 153132.7 | |
| All* | Total formula weight | 153132.7 |
