9WSM
Cryo-EM structure of Sigma28-RNAP open promoter complex from Pseudomonas aeruginosa
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | DNA (54-MER) | polymer | 54 | 16560.6 | 1 | Pseudomonas aeruginosa | ||
| 2 | B (2) | DNA (50-MER) | polymer | 54 | 16622.7 | 1 | Pseudomonas aeruginosa | ||
| 3 | C (E) | DNA-directed RNA polymerase subunit omega | polymer | 88 | 9783.9 | 1 | UniProt (Q9HTM1) Pfam (PF01192) | Pseudomonas aeruginosa | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 4 | D (F) | RNA polymerase sigma factor FliA | polymer | 254 | 28240.9 | 1 | UniProt (P29248) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Pseudomonas aeruginosa | RNA polymerase sigma factor for flagellar operon,Sigma F,Sigma-28 |
| 5 | E, F (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 333 | 36694.6 | 2 | UniProt (O52760) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Pseudomonas aeruginosa | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 6 | G (C) | DNA-directed RNA polymerase subunit beta | polymer | 1357 | 151037.0 | 1 | UniProt (Q51561) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Pseudomonas aeruginosa | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 7 | H (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1399 | 154595.0 | 1 | UniProt (Q9HWC9) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Pseudomonas aeruginosa | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 8 | I (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 9 | J, K (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) |
Sequence modifications
F: 1 - 247 (UniProt: P29248)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Leu -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
| Gly 248 | - | expression tag |
| Ser 249 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 450229.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 450384.4 |
