9WSM

Cryo-EM structure of Sigma28-RNAP open promoter complex from Pseudomonas aeruginosa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (1)	DNA (54-MER)	polymer	54	16560.6	1		Pseudomonas aeruginosa
2	B (2)	DNA (50-MER)	polymer	54	16622.7	1		Pseudomonas aeruginosa
3	C (E)	DNA-directed RNA polymerase subunit omega	polymer	88	9783.9	1	UniProt (Q9HTM1)	Pseudomonas aeruginosa	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
4	D (F)	RNA polymerase sigma factor FliA	polymer	254	28240.9	1	UniProt (P29248)	Pseudomonas aeruginosa	RNA polymerase sigma factor for flagellar operon,Sigma F,Sigma-28
5	E, F (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	333	36694.6	2	UniProt (O52760)	Pseudomonas aeruginosa	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
6	G (C)	DNA-directed RNA polymerase subunit beta	polymer	1357	151037.0	1	UniProt (Q51561)	Pseudomonas aeruginosa	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
7	H (D)	DNA-directed RNA polymerase subunit beta'	polymer	1399	154595.0	1	UniProt (Q9HWC9)	Pseudomonas aeruginosa	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
8	I (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
9	J, K (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

F: 1 - 247 (UniProt: P29248)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Gly 248	-	expression tag
Ser 249	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	450229.3
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	450384.4

*Water molecules are not included.