9WMH

Cryo-EM strucutre of MbpAgo-gDNA-tgRNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Piwi domain-containing protein	polymer	811	93827.4	1	UniProt (H1YCU5)	Mucilaginibacter paludis DSM 18603	Argonaute
2	B (B)	Guide DNA	polymer	18	5641.7	1		synthetic construct
3	C (C)	Target RNA	polymer	19	5926.6	1		synthetic construct
4	D, E, F (A, B)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)

Sequence modifications

A: 1 - 795 (UniProt: H1YCU5)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	105395.6
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	105515.9

*Water molecules are not included.