9WEY

Structure of HCMV UL33 in complex with human Gs protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
2	B (N)	Nanobody35	polymer	144	15969.6	1		Lama glama
3	C (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	393	45568.2	1	UniProt (P63092)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, HiBit fusion	polymer	371	40039.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
5	E (R)	G-protein coupled receptor homolog UL33,G-protein coupled receptor homolog UL33,G-protein coupled receptor homolog UL33, LgBit fusion	polymer	615	68188.7	1		Human betaherpesvirus 5

Sequence modifications

A: 2 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	variant
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation
Ser 366	Ala 366	variant

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

B: 341 - 366 (PDB: 9WEY)
R: 1 - 412 (PDB: 9WEY)
R: 413 - 615 (PDB: 9WEY)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	177496.2
All*	Total formula weight	177496.2

*Water molecules are not included.