9WBG
Structure of human 26S proteasome complexed with midnolin, 19S proteasome with Ubl and Catch domain resolved
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (f) | Maltose/maltodextrin-binding periplasmic protein,Midnolin | polymer | 856 | 92188.2 | 1 | UniProt (P0AEX9) UniProt (Q504T8) | Escherichia coli K-12 | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Midbrain nucleolar protein |
| 2 | B (C) | 26S proteasome regulatory subunit 8 | polymer | 406 | 45694.0 | 1 | UniProt (P62195) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT6,Proteasome 26S subunit ATPase 5,Proteasome subunit p45,Thyroid hormone receptor-interacting protein 1,TRIP1,p45/SUG |
| 3 | C (D) | 26S proteasome regulatory subunit 6B | polymer | 418 | 47426.1 | 1 | UniProt (P43686) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT3,MB67-interacting protein,MIP224,Proteasome 26S subunit ATPase 4,Tat-binding protein 7,TBP-7 |
| 4 | D (V) | 26S proteasome non-ATPase regulatory subunit 3 | polymer | 534 | 61066.5 | 1 | UniProt (O43242) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN3,26S proteasome regulatory subunit S3,Proteasome subunit p58 |
| 5 | E (W) | 26S proteasome non-ATPase regulatory subunit 12 | polymer | 456 | 52979.4 | 1 | UniProt (O00232) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN5,26S proteasome regulatory subunit p55 |
| 6 | F (X) | 26S proteasome non-ATPase regulatory subunit 11 | polymer | 422 | 47526.7 | 1 | UniProt (O00231) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN6,26S proteasome regulatory subunit S9,26S proteasome regulatory subunit p44.5 |
| 7 | G (Y) | 26S proteasome non-ATPase regulatory subunit 6 | polymer | 389 | 45592.3 | 1 | UniProt (Q15008) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN7,26S proteasome regulatory subunit S10,Breast cancer-associated protein SGA-113M,Phosphonoformate immuno-associated protein 4,Proteasome regulatory particle subunit p44S10,p42A |
| 8 | H (Z) | 26S proteasome non-ATPase regulatory subunit 7 | polymer | 324 | 37086.4 | 1 | UniProt (P51665) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN8,26S proteasome regulatory subunit S12,Mov34 protein homolog,Proteasome subunit p40 |
| 9 | I (a) | 26S proteasome non-ATPase regulatory subunit 13 | polymer | 376 | 42995.4 | 1 | UniProt (Q9UNM6) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN9,26S proteasome regulatory subunit S11,26S proteasome regulatory subunit p40.5 |
| 10 | J (b) | 26S proteasome non-ATPase regulatory subunit 4 | polymer | 377 | 40781.6 | 1 | UniProt (P55036) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN10,26S proteasome regulatory subunit S5A,Antisecretory factor 1,AF,ASF,Multiubiquitin chain-binding protein |
| 11 | K (d) | 26S proteasome non-ATPase regulatory subunit 8 | polymer | 350 | 39667.9 | 1 | UniProt (P48556) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN12,26S proteasome regulatory subunit S14,p31 |
| 12 | L (e) | 26S proteasome complex subunit SEM1 | polymer | 70 | 8284.6 | 1 | UniProt (P60896) | Homo sapiens (human) | 26S proteasome complex subunit DSS1,Deleted in split hand/split foot protein 1,Split hand/foot deleted protein 1,Split hand/foot malformation type 1 protein |
| 13 | M (v) | Substrate | polymer | 7 | 613.7 | 1 | Homo sapiens (human) | ||
| 14 | N (U) | 26S proteasome non-ATPase regulatory subunit 1 | polymer | 953 | 105958.2 | 1 | UniProt (Q99460) | Homo sapiens (human) | 26S proteasome regulatory subunit RPN2,26S proteasome regulatory subunit S1,26S proteasome subunit p112 |
| 15 | O (c) | Ubiquitin C-terminal hydrolase PSMD14,Uncharacterized protein | polymer | 590 | 66145.2 | 1 | UniProt (O00487) UniProt (A0ACC5U4E7) | Homo sapiens (human) | 26S proteasome non-ATPase regulatory subunit 14,26S proteasome regulatory subunit RPN11,26S proteasome-associated PAD1 homolog 1 |
| 16 | P (A) | 26S proteasome regulatory subunit 7 | polymer | 433 | 48700.8 | 1 | UniProt (P35998) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT1,Proteasome 26S subunit ATPase 2 |
| 17 | Q (B) | 26S proteasome regulatory subunit 4 | polymer | 440 | 49260.5 | 1 | UniProt (P62191) | Homo sapiens (human) | P26s4,26S proteasome AAA-ATPase subunit RPT2,Proteasome 26S subunit ATPase 1 |
| 18 | R (E) | 26S proteasome regulatory subunit 10B | polymer | 389 | 44241.0 | 1 | UniProt (P62333) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT4,Proteasome 26S subunit ATPase 6,Proteasome subunit p42 |
| 19 | S (F) | 26S proteasome regulatory subunit 6A | polymer | 439 | 49266.5 | 1 | UniProt (P17980) | Homo sapiens (human) | 26S proteasome AAA-ATPase subunit RPT5,Proteasome 26S subunit ATPase 3,Proteasome subunit P50,Tat-binding protein 1,TBP-1 |
| 20 | T (w) | Early growth response protein 1 | polymer | 337 | 37159.6 | 1 | Homo sapiens (human) | EGR-1,AT225,Nerve growth factor-induced protein A,NGFI-A,Transcription factor ETR103,Transcription factor Zif268,Zinc finger protein 225,Zinc finger protein Krox-24 | |
| 21 | CA, U (F, C) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 22 | AA, V, X, Y (E, D, A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 4 | Chemie (ATP) | |||
| 23 | W (c) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 24 | BA, DA, Z (E, F, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) PubChem (888) |
Sequence modifications
f: -379 - -14 (UniProt: P0AEX9)
f: 1 - 468 (UniProt: Q504T8)
c: 1 - 310 (UniProt: O00487)
c: 319 - 554 (UniProt: A0ACC5U4E7)
| PDB | External Database | Details |
|---|---|---|
| Met -387 | - | initiating methionine |
| Gly -386 | - | expression tag |
| His -385 | - | expression tag |
| His -384 | - | expression tag |
| His -383 | - | expression tag |
| His -382 | - | expression tag |
| His -381 | - | expression tag |
| His -380 | - | expression tag |
| Asp -13 | - | linker |
| Tyr -12 | - | linker |
| Asp -11 | - | linker |
| Ile -10 | - | linker |
| Pro -9 | - | linker |
| Thr -8 | - | linker |
| Thr -7 | - | linker |
| Glu -6 | - | linker |
| Asn -5 | - | linker |
| Leu -4 | - | linker |
| Tyr -3 | - | linker |
| Phe -2 | - | linker |
| Gln -1 | - | linker |
| Gly 0 | - | linker |
c: 1 - 310 (UniProt: O00487)
| PDB | External Database | Details |
|---|---|---|
| Glu 311 | - | linker |
| Asn 312 | - | linker |
| Leu 313 | - | linker |
| Tyr 314 | - | linker |
| Phe 315 | - | linker |
| Gln 316 | - | linker |
| Gly 317 | - | linker |
| Ala 318 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| His 555 | - | expression tag |
| His 556 | - | expression tag |
| His 557 | - | expression tag |
| His 558 | - | expression tag |
| His 559 | - | expression tag |
| His 560 | - | expression tag |
| Ser 561 | - | expression tag |
| Ala 562 | - | expression tag |
| Trp 563 | - | expression tag |
| Ser 564 | - | expression tag |
| His 565 | - | expression tag |
| Pro 566 | - | expression tag |
| Gln 567 | - | expression tag |
| Phe 568 | - | expression tag |
| Glu 569 | - | expression tag |
| Lys 570 | - | expression tag |
| Gly 571 | - | expression tag |
| Gly 572 | - | expression tag |
| Gly 573 | - | expression tag |
| Ser 574 | - | expression tag |
| Gly 575 | - | expression tag |
| Gly 576 | - | expression tag |
| Gly 577 | - | expression tag |
| Ser 578 | - | expression tag |
| Gly 579 | - | expression tag |
| Gly 580 | - | expression tag |
| Ser 581 | - | expression tag |
| Ala 582 | - | expression tag |
| Trp 583 | - | expression tag |
| Ser 584 | - | expression tag |
| His 585 | - | expression tag |
| Pro 586 | - | expression tag |
| Gln 587 | - | expression tag |
| Phe 588 | - | expression tag |
| Glu 589 | - | expression tag |
| Lys 590 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 20 |
| Total formula weight | 962634.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3021.5 | |
| All* | Total formula weight | 965656.0 |
