9W74

Cryo-EM structure of the close-packed di-hexasome (CPDH)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, G, K (A, E, K, O)	Histone H3.2	polymer	136	15435.1	4	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F, H, L (B, F, L, P)	Histone H4	polymer	120	13307.5	4	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, I (C, M)	Histone H2A type 1	polymer	165	18087.0	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, J (D, N)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	M (I)	DNA (207-MER)	polymer	207	64788.9	1		Escherichia coli
6	N (J)	DNA (207-MER)	polymer	207	62922.8	1		Escherichia coli

Sequence modifications

A, E, K, O: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

B, F, L, P: 0 - 102 (UniProt: P62799)

PDB	External Database	Details
Ser 103	-	expression tag
Ser 104	-	expression tag
Gly 105	-	expression tag
Leu 106	-	expression tag
Val 107	-	expression tag
Pro 108	-	expression tag
Arg 109	-	expression tag
Gly 110	-	expression tag
Ser 111	-	expression tag
Leu 112	-	expression tag
Glu 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag

C, M: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Met -35	-	initiating methionine
Lys -34	-	expression tag
Glu -33	-	expression tag
Thr -32	-	expression tag
Ala -31	-	expression tag
Ala -30	-	expression tag
Ala -29	-	expression tag
Lys -28	-	expression tag
Phe -27	-	expression tag
Glu -26	-	expression tag
Arg -25	-	expression tag
Gln -24	-	expression tag
His -23	-	expression tag
Met -22	-	expression tag
Asp -21	-	expression tag
Ser -20	-	expression tag
Pro -19	-	expression tag
Asp -18	-	expression tag
Leu -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Arg 99	Gly 100	variant
Ser 123	Ala 124	variant

D, N: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	variant

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	306168.1
All*	Total formula weight	306168.1

*Water molecules are not included.