9W65

Cryo-EM structure of ATP bound state human ABCD3 in inward-facing conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ATP-binding cassette sub-family D member 3,Green fluorescent protein	polymer	914	104526.6	1	UniProt (P28288) UniProt (P42212)	Homo sapiens (human)	70 kDa peroxisomal membrane protein,PMP70
2	B, D (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	C, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
4	F, G (A)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR) PubChem (5997)

Sequence modifications

A: 1 - 659 (UniProt: P28288)

PDB	External Database	Details
Gln 596	Glu 596	engineered mutation
Leu 660	-	linker
Glu 661	-	linker
Val 662	-	linker
Leu 663	-	linker
Phe 664	-	linker
Gln 665	-	linker
Gly 666	-	linker
Pro 667	-	linker
Ala 668	-	linker

A: 669 - 906 (UniProt: P42212)

PDB	External Database	Details
Val 669	Met 1	conflict
Leu 732	Phe 64	conflict
Thr 733	Ser 65	conflict
Lys 874	Ala 206	conflict
Leu 899	His 231	conflict
His 907	-	expression tag
His 908	-	expression tag
His 909	-	expression tag
His 910	-	expression tag
His 911	-	expression tag
His 912	-	expression tag
His 913	-	expression tag
His 914	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	104526.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1836.3
All*	Total formula weight	106362.9

*Water molecules are not included.