9W50

MprF from Pseudomonas aeruginosa mutant- H386C/F389C in nanodisc, C2 symmetry

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Phosphatidylglycerol lysyltransferase	polymer	903	98251.4	2	UniProt (Q9I537)	Pseudomonas aeruginosa PAO1	Lysylphosphatidylglycerol synthase
2	C, D, E, F (A, B)	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	4	Chemie (PGT)

Sequence modifications

A, B: 1 - 881 (UniProt: Q9I537)

PDB	External Database	Details
Met -12	-	initiating methionine
Trp -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
Pro -8	-	expression tag
Gln -7	-	expression tag
Phe -6	-	expression tag
Glu -5	-	expression tag
Lys -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Cys 386	His 386	engineered mutation
Cys 389	Phe 389	engineered mutation
Arg 882	-	expression tag
Asn 883	-	expression tag
Ser 884	-	expression tag
Ser 885	-	expression tag
Ser 886	-	expression tag
Val 887	-	expression tag
Asp 888	-	expression tag
Ala 889	-	expression tag
Leu 890	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	196502.8
Non-Polymers*	Number of molecules	4
	Total formula weight	3004.1
All*	Total formula weight	199506.9

*Water molecules are not included.