9W4S

Cryo-EM structure of hTUT4_mini:hLin28A:pre-let-7g miRNA_UUU, conformation 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Terminal uridylyltransferase 4	polymer	1121	128071.1	1	UniProt (Q5TAX3) Pfam (PF19088) Pfam (PF03828) Pfam (PF01909) Pfam (PF00098)	Homo sapiens (human)	TUTase 4,Zinc finger CCHC domain-containing protein 11
2	B (B)	Protein lin-28 homolog A	polymer	217	23850.1	1	UniProt (Q9H9Z2) Pfam (PF00313) Pfam (PF00098) Pfam (PF21890)	Homo sapiens (human)	Lin-28A,Zinc finger CCHC domain-containing protein 1
3	C (C)	pre-let-7g miRNA	polymer	81	26035.4	1		Homo sapiens
4	D, E, F (A, B)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

A: 202 - 1313 (UniProt: Q5TAX3)

PDB	External Database	Details
Met 201	-	initiating methionine
Leu 1314	-	expression tag
Glu 1315	-	expression tag
His 1316	-	expression tag
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag

B: 1 - 209 (UniProt: Q9H9Z2)

PDB	External Database	Details
Leu 210	-	expression tag
Glu 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	177956.6
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	178152.8

*Water molecules are not included.