9W49

Cryo-EM structure of human kappa opioid receptor -G protein signaling complex bound with U-50488H

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Kappa-type opioid receptor	polymer	314	35658.4	1	UniProt (P41145) Pfam (PF00001)	Homo sapiens (human)	K-OR-1,KOR-1
5	E (S)	scFv16	polymer	255	27318.4	1		Mus musculus
6	F (R)	2-(3,4-dichlorophenyl)-~{N}-methyl-~{N}-[(1~{R},2~{R})-2-pyrrolidin-1-ylcyclohexyl]ethanamide	non-polymer		369.3	1	Chemie (A1LWX)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 54 - 358 (UniProt: P41145)

PDB	External Database	Details
Leu 51	-	expression tag
Gly 52	-	expression tag
Ser 53	-	expression tag
Leu 135	Ile 135	engineered mutation
Leu 359	-	expression tag
Glu 360	-	expression tag
Val 361	-	expression tag
Leu 362	-	expression tag
Phe 363	-	expression tag
Gln 364	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	148981.1
Non-Polymers*	Number of molecules	1
	Total formula weight	369.3
All*	Total formula weight	149350.5

*Water molecules are not included.