9W45

The structure of odorant-bound mouse class II odorant receptor-miniGs complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha	polymer	250	29051.8	1	UniProt (Q5FWY2)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	130	14273.9	1		Lama glama
5	E (R)	Olfactory receptor	polymer	312	35201.2	1	UniProt (Q7TRW7)	Mus musculus (house mouse)
6	F (R)	Methyl cinnamate	non-polymer		162.2	1	Chemie (A1MBB)

Sequence modifications

A: 6 - 194 (UniProt: Q5FWY2)

PDB	External Database	Details
Gly 4	-	expression tag
Ser 5	-	expression tag
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

A: 204 - 394 (UniProt: Q5FWY2)

PDB	External Database	Details
His 195	-	linker
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 235	engineered mutation
Asp 252	Ser 238	engineered mutation
-	Asn 240	deletion
-	Met 241	deletion
-	Val 242	deletion
-	Ile 243	deletion
-	Arg 244	deletion
-	Glu 245	deletion
-	Asp 246	deletion
-	Asn 247	deletion
-	Gln 248	deletion
-	Thr 249	deletion
Leu 272	Pro 258	engineered mutation
Ala 372	Ile 358	engineered mutation
Ile 375	Val 361	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 1 - 312 (UniProt: Q7TRW7)

PDB	External Database	Details
Val 263	Leu 263	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	125806.1
Non-Polymers*	Number of molecules	1
	Total formula weight	162.2
All*	Total formula weight	125968.3

*Water molecules are not included.