9W3K

GPR151-Legobody complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Maltose/maltodextrin-binding periplasmic protein,Immunoglobulin G-binding protein A,Immunoglobulin G-binding protein G	polymer	559	60575.7	1	UniProt (P0AEY0) UniProt (P38507) UniProt (P0A015) UniProt (P06654)	Escherichia coli O157:H7	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,IgG-binding protein A,Staphylococcal protein A,SpA,IgG-binding protein G
2	B (H)	Fab-8D3-2-H-His	polymer	253	27396.7	1		Homo sapiens
3	C (L)	Fab_8D3_L	polymer	239	26317.5	1		Homo sapiens
4	D (A)	G-protein coupled receptor 151	polymer	419	46708.2	1	UniProt (Q8TDV0)	Homo sapiens (human)	G-protein coupled receptor PGR7,GPCR-2037,Galanin receptor 4,Galanin-receptor-like protein,GalRL
5	E (B)	NB6	polymer	127	14057.6	1		Spodoptera frugiperda

Sequence modifications

C: 2 - 369 (UniProt: P0AEY0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Gln 360	Glu 385	conflict
Ala 363	Lys 388	conflict
Phe 364	Asp 389	conflict
Ile 367	Thr 392	conflict
Leu 368	Arg 393	conflict
Met 370	-	linker

C: 371 - 409 (UniProt: P38507)

PDB	External Database	Details
Glu 404	Asp 322	conflict
His 405	Ala 323	conflict
Gly 410	-	linker
Gly 411	-	linker
Ser 412	-	linker
Gly 413	-	linker
Gly 414	-	linker
Ala 415	-	linker
Gly 416	-	linker
Ser 417	-	linker
Gly 418	-	linker

C: 419 - 467 (UniProt: P0A015)

PDB	External Database	Details
Gly 468	-	linker
Gly 469	-	linker
Gly 470	-	linker
Ser 471	-	linker
Gly 472	-	linker
Gly 473	-	linker
Gly 474	-	linker
Ser 475	-	linker
Gly 476	-	linker
Gly 477	-	linker
Ser 478	-	linker

C: 479 - 536 (UniProt: P06654)

PDB	External Database	Details
Gly 537	-	expression tag
Ser 538	-	expression tag
Gly 539	-	expression tag

A: 1 - 221 (UniProt: Q8TDV0)

PDB	External Database	Details
Leu 86	Ser 86	conflict
Trp 127	Leu 127	conflict
Ala 200	Ser 200	conflict

A: 253 - 419 (UniProt: Q8TDV0)

PDB	External Database	Details
Tyr 222	-	linker
Thr 223	-	linker
Leu 224	-	linker
Met 225	-	linker
Ile 226	-	linker
Leu 227	-	linker
Arg 228	-	linker
Leu 229	-	linker
Lys 230	-	linker
Ser 231	-	linker
Val 232	-	linker
Arg 233	-	linker
Leu 234	-	linker
Leu 235	-	linker
Ser 236	-	linker
Gly 237	-	linker
Ser 238	-	linker
Arg 239	-	linker
Glu 240	-	linker
Lys 241	-	linker
Asp 242	-	linker
Arg 243	-	linker
Asn 244	-	linker
Leu 245	-	linker
Arg 246	-	linker
Arg 247	-	linker
Ile 248	-	linker
Thr 249	-	linker
Arg 250	-	linker
Leu 251	-	linker
Val 252	-	linker
Ala 300	Ser 300	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	175055.9
All*	Total formula weight	175055.9

*Water molecules are not included.