9W2U
Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (J) | NADH-ubiquinone oxidoreductase chain 6 | polymer | 175 | 19110.5 | 1 | UniProt (P03924) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit 6 |
| 2 | B (A) | NADH-ubiquinone oxidoreductase chain 3 | polymer | 114 | 12927.3 | 1 | UniProt (Q8WAB4) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit 3 |
| 3 | C (B) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial | polymer | 155 | 17773.9 | 1 | UniProt (P42026) | Bos taurus (domestic cattle) | Complex I-20kD,CI-20kD,NADH-ubiquinone oxidoreductase 20 kDa subunit,PSST subunit |
| 4 | D (C) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial | polymer | 209 | 24547.7 | 1 | UniProt (P23709) | Bos taurus (domestic cattle) | Complex I-30kD,CI-30kD,NADH-ubiquinone oxidoreductase 30 kDa subunit |
| 5 | E (D) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial | polymer | 430 | 49263.4 | 1 | UniProt (P17694) | Bos taurus (domestic cattle) | Complex I-49kD,CI-49kD,NADH-ubiquinone oxidoreductase 49 kDa subunit |
| 6 | F (E) | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial | polymer | 214 | 23655.2 | 1 | UniProt (P04394) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit II,NADH-ubiquinone oxidoreductase 24 kDa subunit |
| 7 | G (F) | NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial | polymer | 432 | 47383.9 | 1 | UniProt (P25708) | Bos taurus (domestic cattle) | NDUFV1,Complex I-51kD,CI-51kD,NADH dehydrogenase flavoprotein 1,NADH-ubiquinone oxidoreductase 51 kDa subunit |
| 8 | H (G) | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | polymer | 699 | 76628.6 | 1 | UniProt (P15690) | Bos taurus (domestic cattle) | Complex I-75kD,CI-75kD |
| 9 | I (H) | NADH-ubiquinone oxidoreductase chain 1 | polymer | 317 | 35557.6 | 1 | UniProt (P03887) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit 1 |
| 10 | J (I) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial | polymer | 176 | 20219.9 | 1 | UniProt (P42028) | Bos taurus (domestic cattle) | Complex I-23kD,CI-23kD,NADH-ubiquinone oxidoreductase 23 kDa subunit,TYKY subunit |
| 11 | K (K) | NADH-ubiquinone oxidoreductase chain 4L | polymer | 97 | 10669.0 | 1 | UniProt (Q6EMS3) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit 4L |
| 12 | L (L) | NADH-ubiquinone oxidoreductase chain 5 | polymer | 605 | 68196.7 | 1 | UniProt (P03920) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit 5 |
| 13 | M (M) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 458 | 51999.6 | 1 | UniProt (P03910) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit 4 |
| 14 | N (N) | NADH-ubiquinone oxidoreductase chain 2 | polymer | 346 | 39143.7 | 1 | UniProt (P03892) | Bos taurus (domestic cattle) | NADH dehydrogenase subunit 2 |
| 15 | O (O) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial | polymer | 320 | 36754.8 | 1 | UniProt (P34942) | Bos taurus (domestic cattle) | Complex I-42kD,CI-42kD,NADH-ubiquinone oxidoreductase 42 kDa subunit |
| 16 | P (P) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial | polymer | 342 | 38932.8 | 1 | UniProt (P34943) | Bos taurus (domestic cattle) | Complex I-39kD,CI-39kD,NADH-ubiquinone oxidoreductase 39 kDa subunit |
| 17 | Q (Q) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial | polymer | 129 | 14918.9 | 1 | UniProt (Q02375) | Bos taurus (domestic cattle) | Complex I-18 kDa,CI-18 kDa,Complex I-AQDQ,CI-AQDQ,NADH-ubiquinone oxidoreductase 18 kDa subunit |
| 18 | R (R) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial | polymer | 96 | 10551.7 | 1 | UniProt (P23934) | Bos taurus (domestic cattle) | Complex I-13kD-A,CI-13kD-A,NADH-ubiquinone oxidoreductase 13 kDa-A subunit |
| 19 | S (S) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 | polymer | 87 | 9970.5 | 1 | UniProt (Q02370) | Bos taurus (domestic cattle) | Complex I-B8,CI-B8,NADH-ubiquinone oxidoreductase B8 subunit |
| 20 | T (T) | Acyl carrier protein, mitochondrial | polymer | 85 | 9846.3 | 1 | UniProt (Q9CR21) | Bos taurus (domestic cattle) | ACP,CI-SDAP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 21 | U (U) | Acyl carrier protein, mitochondrial | polymer | 86 | 9917.4 | 1 | UniProt (P52505) | Bos taurus (domestic cattle) | ACP,CI-SDAP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 22 | V (V) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 | polymer | 115 | 13203.4 | 1 | UniProt (P23935) | Bos taurus (domestic cattle) | Complex I subunit B13,Complex I-13kD-B,CI-13kD-B,NADH-ubiquinone oxidoreductase 13 kDa-B subunit |
| 23 | W (W) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 | polymer | 115 | 13884.1 | 1 | UniProt (Q02366) | Bos taurus (domestic cattle) | Complex I-B14,CI-B14,NADH-ubiquinone oxidoreductase B14 subunit |
| 24 | X (X) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 | polymer | 171 | 19992.9 | 1 | UniProt (P42029) | Bos taurus (domestic cattle) | Complex I-19kD,CI-19kD,Complex I-PGIV,CI-PGIV,NADH-ubiquinone oxidoreductase 19 kDa subunit |
| 25 | Y (t) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 | polymer | 141 | 14666.9 | 1 | UniProt (Q8HXG6) | Bos taurus (domestic cattle) | Complex I-B14.7,CI-B14.7,NADH-ubiquinone oxidoreductase subunit B14.7 |
| 26 | Z (Z) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 | polymer | 141 | 16421.0 | 1 | UniProt (Q95KV7) | Bos taurus (domestic cattle) | Cell death regulatory protein GRIM-19,Complex I-B16.6,CI-B16.6,Gene associated with retinoic-interferon-induced mortality 19 protein,GRIM-19,NADH-ubiquinone oxidoreductase B16.6 subunit |
| 27 | AA (a) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 | polymer | 70 | 8117.4 | 1 | UniProt (Q02377) | Bos taurus (domestic cattle) | Complex I-MWFE,CI-MWFE,NADH-ubiquinone oxidoreductase MWFE subunit |
| 28 | BA (b) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 | polymer | 83 | 9226.7 | 1 | UniProt (Q02371) | Bos taurus (domestic cattle) | Complex I-B9,CI-B9,NADH-ubiquinone oxidoreductase B9 subunit |
| 29 | CA (c) | NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial | polymer | 49 | 5836.7 | 1 | UniProt (Q02376) | Bos taurus (domestic cattle) | Complex I-KFYI,CI-KFYI,NADH-ubiquinone oxidoreductase KFYI subunit |
| 30 | DA (d) | NADH dehydrogenase [ubiquinone] 1 subunit C2 | polymer | 119 | 13986.1 | 1 | UniProt (Q02827) | Bos taurus (domestic cattle) | Complex I-B14.5b,CI-B14.5b,NADH-ubiquinone oxidoreductase subunit B14.5b |
| 31 | EA (e) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 | polymer | 99 | 11835.7 | 1 | UniProt (Q02379) | Bos taurus (domestic cattle) | Complex I-15 kDa,CI-15 kDa,NADH-ubiquinone oxidoreductase 15 kDa subunit |
| 32 | FA (f) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 | polymer | 57 | 6978.1 | 1 | UniProt (Q02378) | Bos taurus (domestic cattle) | Complex I-MNLL,CI-MNLL,NADH-ubiquinone oxidoreductase MNLL subunit |
| 33 | GA (g) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial | polymer | 100 | 11897.3 | 1 | UniProt (Q8HXG5) | Bos taurus (domestic cattle) | Complex I-ESSS,CI-ESSS,NADH-ubiquinone oxidoreductase ESSS subunit |
| 34 | HA (h) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial | polymer | 138 | 16297.9 | 1 | UniProt (Q02380) | Bos taurus (domestic cattle) | Complex I-SGDH,CI-SGDH,NADH-ubiquinone oxidoreductase SGDH subunit |
| 35 | IA (u) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 | polymer | 128 | 15444.9 | 1 | UniProt (Q02367) | Bos taurus (domestic cattle) | Complex I-B17,CI-B17,NADH-ubiquinone oxidoreductase B17 subunit |
| 36 | JA (j) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial | polymer | 71 | 8385.2 | 1 | UniProt (Q02374) | Bos taurus (domestic cattle) | Complex I-AGGG,CI-AGGG,NADH-ubiquinone oxidoreductase AGGG subunit |
| 37 | KA (k) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 | polymer | 81 | 9229.5 | 1 | UniProt (Q02365) | Bos taurus (domestic cattle) | Complex I-B12,CI-B12,NADH-ubiquinone oxidoreductase B12 subunit |
| 38 | LA (l) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial | polymer | 156 | 18599.8 | 1 | UniProt (Q02372) | Bos taurus (domestic cattle) | Complex I-ASHI,CI-ASHI,NADH-ubiquinone oxidoreductase ASHI subunit |
| 39 | MA (m) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 | polymer | 128 | 15075.1 | 1 | UniProt (P48305) | Bos taurus (domestic cattle) | Complex I-B15,CI-B15,NADH-ubiquinone oxidoreductase B15 subunit |
| 40 | NA (n) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 | polymer | 172 | 21134.1 | 1 | UniProt (Q02369) | Bos taurus (domestic cattle) | Complex I-B22,CI-B22,NADH-ubiquinone oxidoreductase B22 subunit |
| 41 | OA (o) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 | polymer | 122 | 14978.1 | 1 | UniProt (Q02368) | Bos taurus (domestic cattle) | Complex I-B18,CI-B18,NADH-ubiquinone oxidoreductase B18 subunit |
| 42 | PA (p) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 | polymer | 173 | 20701.5 | 1 | UniProt (Q02373) | Bos taurus (domestic cattle) | Complex I-PDSW,CI-PDSW,NADH-ubiquinone oxidoreductase PDSW subunit |
| 43 | QA (q) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 | polymer | 145 | 17115.5 | 1 | UniProt (O97725) | Bos taurus (domestic cattle) | Complex I-B17.2,CIB17.2,NADH-ubiquinone oxidoreductase subunit B17.2 |
| 44 | RA (v) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 | polymer | 113 | 12590.4 | 1 | UniProt (Q05752) | Bos taurus (domestic cattle) | Complex I-B14.5a,CI-B14.5a,NADH-ubiquinone oxidoreductase subunit B14.5a |
| 45 | SA (s) | NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial | polymer | 45 | 5392.9 | 1 | UniProt (P56181) | Bos taurus (domestic cattle) | Complex I-9kD,CI-9kD,NADH-ubiquinone oxidoreductase 9 kDa subunit,Renal carcinoma antigen NY-REN-4 |
| 46 | FB, LB, NB, TA, TB... (H, K, L, A, M...) | N-FORMYLMETHIONINE | non-polymer | 177.2 | 6 | Chemie (FME) PubChem (439750) | |||
| 47 | HB, IB, KC, OC, RB... (H, I, Z, d, L...) | 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 790.1 | 11 | Chemie (PC1) | |||
| 48 | AB, BB, CB, JB, KB... (F, G, I, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 49 | DB, YA (G, E) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 50 | ZA (F) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) PubChem (643976) | |||
| 51 | EB (G) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) PubChem (813) | |||
| 52 | FC, GB, GC, HC, IC... (t, H, b, K, d...) | 1,2-Distearoyl-sn-glycerophosphoethanolamine | non-polymer | 748.1 | 18 | Chemie (3PE) | |||
| 53 | AC, MC, PB, PC, QC... (O, d, L, h, v) | CARDIOLIPIN | non-polymer | 1464.0 | 6 | Chemie (CDL) | |||
| 54 | SB (L) | CHOLIC ACID | non-polymer | 408.6 | 1 | Chemie (CHD) PubChem (221493) | |||
| 55 | BC (O) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 56 | CC (O) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 57 | DC (P) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 58 | EC (R) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) PubChem (32051) | |||
| 59 | WC (o) | MYRISTIC ACID | non-polymer | 228.4 | 1 | Chemie (MYR) PubChem (11005) |
Sequence modifications
A: 2 - 115 (UniProt: Q8WAB4)
D: 1 - 430 (UniProt: P17694)
O: 1 - 320 (UniProt: P34942)
T: 4 - 88 (UniProt: Q9CR21)
t: 1 - 140 (UniProt: Q8HXG6)
u: 1 - 127 (UniProt: Q02367)
v: 1 - 112 (UniProt: Q05752)
s: 31 - 75 (UniProt: P56181)
| PDB | External Database | Details |
|---|---|---|
| Ala 82 | Thr 82 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 129 | Gln 162 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Lys 255 | Asn 278 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 9 | Asp 77 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
| PDB | External Database | Details |
|---|---|---|
| Asp 57 | Glu 90 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 45 |
| Total formula weight | 948960.9 | |
| Non-Polymers* | Number of molecules | 57 |
| Total formula weight | 36956.5 | |
| All* | Total formula weight | 985917.4 |
