9W0U

CryoEM structure of the T2R46 in complex with tangeretin and heterotrimeric G protein complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40458.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Taste receptor type 2 member 46	polymer	363	41557.2	1	UniProt (P59540) Pfam (PF05296)	Homo sapiens (human)	T2R46,Taste receptor type 2 member 54,T2R54
5	E (R)	5,6,7,8-tetramethoxy-2-(4-methoxyphenyl)chromen-4-one	non-polymer		372.4	1	Chemie (A1EUI) PubChem (68077)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 1 - 304 (UniProt: P59540)

PDB	External Database	Details
Met -21	-	initiating methionine
Lys -20	-	expression tag
Thr -19	-	expression tag
Ile -18	-	expression tag
Ile -17	-	expression tag
Ala -16	-	expression tag
Leu -15	-	expression tag
Ser -14	-	expression tag
Tyr -13	-	expression tag
Ile -12	-	expression tag
Phe -11	-	expression tag
Cys -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Phe -7	-	expression tag
Ala -6	-	expression tag
Asp -5	-	expression tag
Tyr -4	-	expression tag
Lys -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Leu 63	Val 63	conflict
Phe 305	-	expression tag
Leu 306	-	expression tag
Glu 307	-	expression tag
Val 308	-	expression tag
Leu 309	-	expression tag
Phe 310	-	expression tag
Gln 311	-	expression tag
Gly 312	-	expression tag
Pro 313	-	expression tag
Trp 314	-	expression tag
Ser 315	-	expression tag
His 316	-	expression tag
Pro 317	-	expression tag
Gln 318	-	expression tag
Phe 319	-	expression tag
Glu 320	-	expression tag
Lys 321	-	expression tag
Gly 322	-	expression tag
Gly 323	-	expression tag
Gly 324	-	expression tag
Ser 325	-	expression tag
Gly 326	-	expression tag
Gly 327	-	expression tag
Gly 328	-	expression tag
Ser 329	-	expression tag
Gly 330	-	expression tag
Gly 331	-	expression tag
Ser 332	-	expression tag
Ala 333	-	expression tag
Trp 334	-	expression tag
Ser 335	-	expression tag
His 336	-	expression tag
Pro 337	-	expression tag
Gln 338	-	expression tag
Phe 339	-	expression tag
Glu 340	-	expression tag
Lys 341	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128620.8
Non-Polymers*	Number of molecules	1
	Total formula weight	372.4
All*	Total formula weight	128993.2

*Water molecules are not included.