9W0P

CryoEM structure of T2R14 in complex with chlorhexidine and heterotrimeric G protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40458.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Soluble cytochrome b562,Taste receptor type 2 member 14	polymer	483	54569.5	1	UniProt (P0ABE7) UniProt (Q9NYV8) Pfam (PF05296)	Escherichia coli	Cytochrome b-562,T2R14,Taste receptor family B member 1,TRB1
5	E (R)	1-[6-[azanylidene-[[azanylidene-[[(4-chlorophenyl)amino]methyl]-$l^{4}-azanyl]methyl]-$l^{4}-azanyl]hexyl]-3-[~{N}-(4-chlorophenyl)carbamimidoyl]guanidine	non-polymer		505.4	1	Chemie (XC9)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: -105 - 0 (UniProt: P0ABE7)

PDB	External Database	Details
Met -127	-	initiating methionine
Lys -126	-	expression tag
Thr -125	-	expression tag
Ile -124	-	expression tag
Ile -123	-	expression tag
Ala -122	-	expression tag
Leu -121	-	expression tag
Ser -120	-	expression tag
Tyr -119	-	expression tag
Ile -118	-	expression tag
Phe -117	-	expression tag
Cys -116	-	expression tag
Leu -115	-	expression tag
Val -114	-	expression tag
Phe -113	-	expression tag
Ala -112	-	expression tag
Asp -111	-	expression tag
Tyr -110	-	expression tag
Lys -109	-	expression tag
Asp -108	-	expression tag
Asp -107	-	expression tag
Asp -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	Arg 128	conflict

R: 1 - 317 (UniProt: Q9NYV8)

PDB	External Database	Details
Glu 318	-	expression tag
Phe 319	-	expression tag
Leu 320	-	expression tag
Glu 321	-	expression tag
Val 322	-	expression tag
Leu 323	-	expression tag
Phe 324	-	expression tag
Gln 325	-	expression tag
Gly 326	-	expression tag
Pro 327	-	expression tag
Trp 328	-	expression tag
Ser 329	-	expression tag
His 330	-	expression tag
Pro 331	-	expression tag
Gln 332	-	expression tag
Phe 333	-	expression tag
Glu 334	-	expression tag
Lys 335	-	expression tag
Gly 336	-	expression tag
Gly 337	-	expression tag
Gly 338	-	expression tag
Ser 339	-	expression tag
Gly 340	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Ser 346	-	expression tag
Ala 347	-	expression tag
Trp 348	-	expression tag
Ser 349	-	expression tag
His 350	-	expression tag
Pro 351	-	expression tag
Gln 352	-	expression tag
Phe 353	-	expression tag
Glu 354	-	expression tag
Lys 355	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	141633.1
Non-Polymers*	Number of molecules	1
	Total formula weight	505.4
All*	Total formula weight	142138.6

*Water molecules are not included.